Bovine Serum Albumin

heat shock fraction, protease free, low endotoxin, suitable for cell culture, pH 7, ≥98%

Albumin bovine serum, Bovine albumin, BSA
CAS Number:
EC Number:
MDL number:

Quality Level

biological source


product line





lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation


poly bottle of


USA origin


cell culture | mammalian: suitable


protease, none detected
≤1.0 EU/mg endotoxin






water: soluble (40 mg/ml)

UniProt accession no.

storage temp.


Gene Information

bovine ... ALB(280717)

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General description

Bovine serum albumin (BSA), a 66 kDa protein, belongs to the family of serum albumins. It is an α-helical, non-glycosylated and globular protein, that has 17-disulfide bonds.


Bovine Serum Albumin has been used:
  • as a component of cell culture media in cochlear explant culture
  • organotypic cultures and embryo culture
  • as a component of phosphate-buffered saline (PBS) in immunostaining of human induced pluripotent stem cell (hiPSC)


1, 5, 25, 100 g in poly bottle

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the most vital constituent of the cell culture media, which aids embryonic stem cells (hESC) differentiation. It acts as a blocking agent in enzyme linked immunosorbent assay (ELISA) to inhibit non-specific binding of antigens and antibodies to the microtiter plates.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Tested for use in cell culture
  • Protease-free
  • Low endotoxin

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.


11 - Combustible Solids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Kanamycin damages early postnatal, but not adult spiral ganglion neurons
Gao K, et al.
Neurotoxicity Research, 32(4), 603-613 (2017)
An immortalized microglial cell line (Mocha) derived from rat cochlea
Seigel GM, et al.
Molecular and Cellular Neurosciences, 85, 202-210 (2017)
Paternal breed effects on expression of IGF-II, BAK1 and BCL2-L1 in bovine preimplantation embryos
Valleh MV, et al.
Zygote, 23(5), 712-721 (2015)
Yunyao Xie et al.
Stem cell research, 26, 84-94 (2017-12-23)
The use of human induced pluripotent stem cell (hiPSC)-derived neuronal cultures to study the mechanisms of neurological disorders is often limited by low efficiency and high variability in differentiation of functional neurons. Here we compare the functional properties of neurons...
Dalian Ding et al.
PloS one, 8(11), e79817-e79817 (2013-11-14)
Mefloquine is widely used for the treatment of malaria. However, this drug is known to induce neurological side effects including depression, anxiety, balance disorder, and sensorineural hearing loss. Yet, there is currently no treatment for these side effects. In this...
This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.
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