Regeneration is generally accomplished by washing with high ionic strength buffer or decreasing/increasing pH. The resin is then re-equilibrated in binding buffer.
A50120
DEAE–Sephadex®
Synonym(s):
Diethylaminoethyl Sephadex®
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50 G
$598.00
$598.00
Estimated to ship onDecember 08, 2025Details
About This Item
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form
powder
Quality Level
technique(s)
protein purification: suitable
matrix
cross-linked dextran
bead size
40-125 μm (dry)
pore size
~200,000 Da exclusion limit
pH
2—12
capacity
3-4 meq/g binding capacity
Related Categories
1 of 4
This Item | DFF100 | DCL6B100 | A25120 |
|---|---|---|---|
| matrix cross-linked dextran | matrix 6% cross-linked agarose | matrix 6% cross-linked agarose | matrix cross-linked dextran |
| pore size ~200,000 Da exclusion limit | pore size ~4,000,000 Da exclusion limit | pore size ~4,000,000 Da exclusion limit | pore size ~30,000 Da exclusion limit |
| capacity 3-4 meq/g binding capacity | capacity 110-160 μeq/mL binding capacity(gel volume) | capacity 130-170 μeq/mL binding capacity (gel volume)(gel volume) | capacity 3-4 meq/g ion exchange capacity |
| form powder | form suspension | form suspension | form powder |
| technique(s) protein purification: suitable | technique(s) affinity chromatography: suitable | technique(s) affinity chromatography: suitable | technique(s) activity assay: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
A50120-100G′s updated product number is GE17-0180-01
A50120-500G′s updated product number is GE17-0180-02
A50120-500G′s updated product number is GE17-0180-02
Application
DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins. In has been used to identify and label an insulin activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients.
Legal Information
Sephadex is a registered trademark of Cytiva
comparable product
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Misook Kim et al.
Biochimica et biophysica acta, 1770(12), 1627-1635 (2007-10-09)
Two cysteine proteases, GP2 and GP3, have been isolated from ginger rhizomes (Zingiber officinale). GP2 is virtually identical to a previously identified ginger protease GPII [K.H. Choi, and R.A. Laursen, Amino-acid sequence and glycan structures of cysteine proteases with proline
Clovis R Nakaie et al.
Analytical biochemistry, 318(1), 39-46 (2003-06-05)
This report demonstrates that due to the presence of residual reactive sites in their matrices, classical diethylaminoethyl-attaching commercial anion-exchanger resins such as DEAE-MacroPrep and DEAE-Sephadex A50 supports can be used for peptide synthesis. Moreover, due to the high stability of
M A Zaval'nyĭ et al.
Voprosy virusologii, (5)(5), 583-589 (1980-09-01)
Primary cells of chick embryos (CEC), new-born piglet kidneys (NPK), and green monkey kidney (GMK) obtained by the conventional method of tissue trypsinization were shown to grow satisfactorily on microcarriers of various types. CEC formed a confluent monolayer on Supperbead
E Okuno et al.
Biochimica et biophysica acta, 841(1), 112-119 (1985-07-26)
Quinolinic acid phosphoribosyltransferase (EC 2.4.2.19) was purified 3600-fold from rat liver and 280-fold from rat brain. Kinetic analyses (Km = 12 microM for the substrate quinolinic acid and Km = 23 microM for the cosubstrate phosphoribosylpyrophosphate), physicochemical properties of the
P K Farmer et al.
Biochimica et biophysica acta, 1434(1), 6-17 (1999-11-11)
A calmodulin-like domain protein kinase (DcCPK1, previously designated CDPK431) cloned from carrot (Daucus carota L.) was expressed at high levels in Escherichia coli and partially purified. Ca(2+)-induced gel mobility shift and (45)Ca(2+) ligand binding assays confirmed that recombinant DcCPK1 binds
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How can Product A50120, DEAE-Sephadex® resin be regenerated?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What procedures are used for elution when using Product A50120, DEAE-Sephadex® resin?
1 answer-
For DEAE Sephadex, elution is achieved using either an increasing salt gradient or a decreasing pH gradient.
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What is the procedure for swelling Product A50120, DEAE-Sephadex® resin?
1 answer-
Weigh out the required amount of dry powder and suspend it in the buffer of choice. Although the swelling volume will be specific to the binding buffer used, generally 1 gram of powder will swell to 25 mL final volume of medium. Excess binding buffer should be used. Swell the resin for 1-2 days at room temperature or 2 hours at 100°C over boiling water. After swelling, wash the resin on a Buchner funnel with binding buffer. Prepare a slurry with 75% swelled medium/25% binding buffer.
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How can Product A50120, DEAE-Sephadex® resin, be stored?
1 answer-
Store swollen medium refrigerated in the presence of a preservative such as 20% Ethanol. Sodium azide or thimerosal should not be used.
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