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A5102

Sigma-Aldrich

Anti-Aurora B antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Anti-AIM-1, Anti-AIR-2 Kinase, Anti-AIRK-2
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 41 kDa

species reactivity

rat, mouse, human

packaging

antibody small pack of 25 μL

technique(s)

immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:50 using HeLa cells
microarray: suitable
western blot: 1:1,000 using nuclei-enriched fraction of mouse NIH-3T3 cells
western blot: 1:1,000 using using PC-12 rat phaeochromocytoma cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AURKB(9212)
mouse ... Aurkb(20877)
rat ... Aurkb(114592)

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This Item
A1231SAB1300093WH0009212M3
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

antibody form

purified immunoglobulin

clone

polyclonal

clone

35C1, monoclonal

clone

polyclonal

clone

6H7, monoclonal

form

buffered aqueous solution

form

PBS solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen 41 kDa

mol wt

antigen ~46 kDa

mol wt

-

mol wt

-

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General description

Aurora B (AIRK2, AIR-2 kinase, AIM-1) is a serine/threonine kinase. Aurora B is evolutionally conserved from yeast to human. The Drosophila serine/threonine protein kinase Aurora and the S. cerevisiae IpI1 kinase are highly homologous to human Aurora B.

Specificity

Anti-Aurora B recognizes human, mouse, and rat Aurora B/AIR-2 Kinase enzymes. Detection of the Aurora B band by immunoblotting is specifically inhibited with the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acid residues 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH.

Application

Anti-Aurora B antibody has been used in:
  • in immunoblotting
  • in immunoprecipitation
  • in immunofluorescence
  • in immunofluorescence staining
  • in western blotting

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Rabbit polyclonal anti-Aurora B antibody is used to tag Aurora B/AIR-2 Kinase for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Aurora B/AIR-2 Kinase in chromosome segregation, cytokinesis, and cancer development.

Biochem/physiol Actions

Aurora B plays key roles in chromosome segregation, cytokinesis, and cancer development. It also plays a role in chromosomal condensation by phosphorylating the H3 histone. In C. elegans, Aurora-B is required for normal localization and function of the ZEN-4/CeMKLP (Kinesin like protein/mitotic kinesin-like protein), a kinesin-related protein essential for completion of cytokinesis. Loss of the Aurora B kinase results in chromosome segregation defects and failures in cytokinesis. Aurora B displays a localization pattern typical of chromosomal passenger proteins as the inner centromeric protein (INCENP), TD-60 and Survivin. INCENP and Survivin interact directly with Aurora B. Chromosomal passenger proteins undergo dynamic redistribution during mitosis. They localize at centromers during prometaphase and relocate to midzone microtubules and midbodies during anaphase and telophase. The mRNA and protein levels of Aurora B are induced during G2M and decrease rapidly after the end of mitosis. Levels of Aurora B are increased in several human cancer cell lines.

Target description

Auroa B is a member of a family of Auora kinases that function for proper chromosomal segregation during mitosis. Auora B localizes specifically to the kinetochores of microtubules.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Aurora B regulates MCAK at the mitotic centromere
Andrews P D, et al.
Developmental Cell, 6(2), 253-268 (2004)
Shi-Zheng Wu et al.
Cell biochemistry and function, 35(2), 113-123 (2017-02-25)
It has been reported that CXCR4-overexpressing mesenchymal stem cells (MSCCX4 ) can repair heart tissue post myocardial infarction. This study aims to investigate the MSCCX4-derived paracrine cardio-protective signaling in the presence of myocardial infarction. Mesenchymal stem cells (MSCs) were divided
Yu-Chu Wang et al.
Experimental & molecular medicine, 50(6), 70-70 (2018-06-10)
Heterogeneous nuclear ribonucleoprotein (hnRNP) Q1, an RNA-binding protein, has been implicated in many post-transcriptional processes, including RNA metabolism and mRNA splicing and translation. However, the role of hnRNP Q1 in tumorigenesis remains unclear. We previously performed RNA immunoprecipitation (RIP)-seq analysis
Validating Aurora B as an anti-cancer drug target
Girdler F, et al.
Journal of Cell Science, 119(17), 3664-3675 (2006)
Combining neuropeptide Y and mesenchymal stem cells reverses remodeling after myocardial infarction
Wang Y, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 298(1), H275-H286 (2009)

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