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A5153

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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MDL number:
NACRES:
NA.46

biological source

goat

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:7,000-1:21,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
A9316A3562A4656
biological source

goat

biological source

goat

biological source

goat

biological source

goat

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

Affinity isolated antigen specific antibody is obtained from goat anti-mouse IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, that do not specifically bind to mouse IgG. Goat anti-Mouse IgG is conjugated to Sigma Alkaline Phosphatase, Type VII-S (Product No. P 5521) by protein crosslinking with 0.2% glutaraldehyde. Anti-Mouse IgG (Whole Molecule) is determined to be immunospecific for mouse IgG by immunoelectrophoresis (IEP), versus mouse IgG and normal mouse serum, prior to conjugation. Prior to conjugation, the antibody is found to be reactive with mouse IgG1, IgG2a, IgG2b, and IgG by Ouchterlony Double Diffusion (ODD). Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation.

Specificity

Binds all mouse Igs.
mouse IgG1, IgG2a, IgG2b, and IgG

Immunogen

Purified mouse IgG

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at μg/ml in TBS/Tween containing final concentration of 0.5M NaCl.
Western blot analysis of nuclear or mitochondrial protein extracts were performed using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary antibody at a 1:5000 dilution in 5%BSA/TBS for 1 hour at room temperature. 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium (Sigma) was used for the substrate.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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Xian Fu et al.
mBio, 8(5) (2017-09-07)
Methionine sulfoxide reductase A (MsrA) is an antioxidant enzyme found in all domains of life that catalyzes the reduction of methionine-
Lana J McMillan et al.
Applied and environmental microbiology, 82(2), 538-548 (2015-11-08)
Soluble inorganic pyrophosphatases (PPAs) that hydrolyze inorganic pyrophosphate (PPi) to orthophosphate (Pi) are commonly used to accelerate and detect biosynthetic reactions that generate PPi as a by-product. Current PPAs are inactivated by high salt concentrations and organic solvents, which limits
Siti Aisyah Abdul Ahmad et al.
Virology journal, 14(1), 229-229 (2017-11-23)
The rapid rise and spread in dengue cases, together with the unavailability of safe vaccines and effective antiviral drugs, warrant the need to discover and develop novel anti-dengue treatments. In this study the antiviral activity of geraniin, extracted from the
Dhaval K Shah et al.
International journal of pharmaceutics, 465(1-2), 228-238 (2014-02-11)
To facilitate the development of an inverse targeting strategy, where anti-topotecan antibodies are administered to prevent systemic toxicity following intraperitoneal topotecan, a pharmacokinetic/toxicodynamic (PK/TD) model was developed and evaluated. The pharmacokinetics of 8C2, a monoclonal anti-topotecan antibody, were assessed following
Valerie Odon et al.
Nucleic acids research, 47(15), 8061-8083 (2019-07-06)
Zinc finger antiviral protein (ZAP) is a powerful restriction factor for viruses with elevated CpG dinucleotide frequencies. We report that ZAP similarly mediates antiviral restriction against echovirus 7 (E7) mutants with elevated frequencies of UpA dinucleotides. Attenuation of both CpG-

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