A5228

Sigma-Aldrich

Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal

clone 1A4, purified from hybridoma cell culture

Synonym(s):
Monoclonal Anti-Actin, α-Smooth Muscle, Acta2 Antibody, SMA, Alpha Smooth Muscle Actin Antibody Sigma, Sma Antibody, SMA Antibody - Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A4, monoclonal

form

PBS solution

mol wt

antigen ~42 kDa

species reactivity

human, frog, sheep, chicken, goat, bovine, rat, guinea pig, mouse, canine, rabbit, snake

packaging

antibody small pack of 25 μL

application(s)

immunocytochemistry: suitable using smooth muscle cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells
indirect ELISA: suitable
indirect immunofluorescence: 5-10 μg/mL using blood vessels in formalin-fixed, paraffin-embedded human tonsil or appendix tissue sections
microarray: suitable
western blot: suitable using smooth muscle cells

isotype

IgG2a

conjugate

unconjugated

Featured Industry

Research Pathology

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

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General description

The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.
Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha actins (skeletal, cardiac, smooth), one beta actin (beta-non-muscle) and two gamma actins (gamma smooth muscle and gamma non-muscle).
Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the 1A4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the NH2 terminal synthetic decapeptide of a smooth muscle actin, coupled to keyhole limpet hemocyanin (KLH).

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

Application

Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal has been used in immunofluorescence staining, immunohistochemistry, immunoblotting, immunocytochemistry and enzyme linked immunosorbent assay (ELISA).
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.

Packaging

200 μL in glass bottle

Biochem/physiol Actions

Actin and myosin are constituents of many cells types and are involved in a myriad of cellular processes including locomotion, cytokinesis, secretion, cytoplasmic streaming and phagocytosis. It has been shown that the relative proportion of actin isoforms are different in smooth muscles of different organs and change within the same population of smooth muscle cells during development, pathological situations and different culture conditions. The actin in cells of various species and tissue origin are very similar in their immunological and physical properties.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Special characteristics of culturing mature human bladder smooth muscle cells on human amniotic membrane as a suitable matrix
Sharifiaghdas F, et al.
Urology, 6(4), 283-288 (2009)
Up-regulation of alpha-smooth muscle actin in cardiomyocytes from non-hypertrophic and non-failing transgenic mouse hearts expressing N-terminal truncated cardiac troponin I
Kern S, et al.
FEBS Open Bio, 4(8), 11-17 (2014)
Jennifer J P Collins et al.
American journal of physiology. Lung cellular and molecular physiology, 299(6), L852-L860 (2010-10-12)
Bronchopulmonary dysplasia is associated with chorioamnionitis and fetal lung inflammation. Ureaplasma species are the bacteria most frequently isolated from chorioamnionitis. Very chronic ureaplasma colonization of amniotic fluid causes low-grade lung inflammation and functional lung maturation in fetal sheep. Less is...
Neuron-like differentiation of adipose tissue-derived stromal cells and vascular smooth muscle cells
Ning H, et al.
Differentiation, 74(9-10), 510-518 (2006)
Induction of renal fibrotic genes by TGF-beta1 requires EGFR activation, p53 and reactive oxygen species
Samarakoon R, et al.
Cellular Signalling, 25(11), 2198-2209 (2013)

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