Skip to Content
MilliporeSigma
All Photos(1)

Documents

A5354

Sigma-Aldrich

Ampicillin

Ready Made Solution, 100 mg/mL, 0.2 μm filtered

Sign Into View Organizational & Contract Pricing

Synonym(s):
Aminobenzylpenicillin
UNSPSC Code:
51280000
NACRES:
NA.76

biological source

microbial

Quality Level

sterility

0.2 μm filtered

form

liquid

autoignition temp.

301 °C

storage condition

(Tightly closed. Keep locked up or in an area accessible only to qualified or authorized )

concentration

100 mg/mL

color

colorless to light yellow

solubility

DMSO: 100 mg/mL

suitability

suitable (for antibacterial use in culture media)

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

mode of action

cell wall synthesis | interferes

shipped in

dry ice

storage temp.

−20°C

SMILES string

CC1(C(N2C(S1)C(C2=O)NC(=O)C(C3=CC=CC=C3)N)C(=O)[O-])C.[Na+]

InChI

1S/C16H19N3O4S.Na/c1-16(2)11(15(22)23)19-13(21)10(14(19)24-16)18-12(20)9(17)8-6-4-3-5-7-8;/h3-7,9-11,14H,17H2,1-2H3,(H,18,20)(H,22,23);/q;+1/p-1/t9-,10-,11+,14-;/m1./s1

InChI key

KLOHDWPABZXLGI-YWUHCJSESA-M

Related Categories

General description

Ampicillin, a member of the extended-spectrum β-lactam family, is a semisynthetic derivative of penicillin widely employed as a versatile broad-spectrum antibiotic. Its mechanism of action involves inhibiting bacterial cell wall synthesis by binding to penicillin-binding proteins (PBPs), disrupting the crucial peptidoglycan synthesis process essential for bacterial cell wall formation. This antibiotic exhibits efficacy against a diverse range of bacteria, including Gram-positive and Gram-negative strains like E. coli, β-lactam sensitive vancomycin-resistant Enterococcus (VRE), Staphylococcus aureus, and Streptococcus pneumoniae.In scientific research, ampicillin assumes a pivotal role, particularly in microbiological, biochemical, and cell biology investigations. Its application in laboratories extends to the exploration of antibiotic resistance and penetration limitations, the study of synergistic interactions among multiple antibiotics, and serving as a vital component for selecting and maintaining recombinant plasmids in E. coli. Ampicillin in a convenient ready-to-use 100 mg/mL in DMSO solution is useful in the selection of bacteria cells with specific resistance.
Chemical structure: ß-lactam

Application

Ampicillin is used to select bacteria cells with specific resistance during general microbiology studies. It is used to study antibiotic resistance and penetration limitations, the synergy between multiple antibiotics, certain bloodstream infections, and has been used to develop PCR assays to detect resistance genes in cerebrospinal fluid .

Biochem/physiol Actions

Mode of Action: This is a ß-lactam antibiotic that inhibits bacterial cell-wall synthesis by inactivating transpeptidases on the inner surface of the bacterial cell membrane.Mode of Resistance: Administration with ß-lactamase cleaves the ß-lactam ring of Ampicillin and inactivates it.

Antimicrobial Spectrum: Includes both gram-positive (similar to benzylpenicillin) and gram-negative bacteria (similar to tetracyclines and chloramphenicol.

Features and Benefits

  • High-quality antibiotic suitable for multiple research applications
  • Ideal for Cell Biology and Biochemical research.

Other Notes

For additional information on our range of Biochemicals, please complete this form.
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefullyresealed and kept upright to prevent leakage.

Related product

Product No.
Description
Pricing

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

wgk_germany

WGK 2

flash_point_f

closed cup

flash_point_c

closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 6

1 of 6

Chloramphenicol Ready Made Solution, 100 mg/mL in ethanol: isopropanol (95:5), ≥98% (HPLC)

Sigma-Aldrich

R4408

Chloramphenicol

Ampicillin sodium United States Pharmacopeia (USP) Reference Standard

USP

1033203

Ampicillin sodium

Sigma-Aldrich

Sigma-Aldrich

C1613

Carbenicillin

LB Broth (Miller) Liquid microbial growth medium

Sigma-Aldrich

L2542

LB Broth (Miller)

Ampicillin trihydrate 900-1050 μg/mg anhydrous basis (HPLC)

Sigma-Aldrich

A6140

Ampicillin trihydrate

Maria Chiara Lionetti et al.
Journal of clinical medicine, 9(8) (2020-08-14)
Melanoma is one of the most aggressive and highly resistant tumors. Cell plasticity in melanoma is one of the main culprits behind its metastatic capabilities. The detailed molecular mechanisms controlling melanoma plasticity are still not completely understood. Here we combine
J N Anderl et al.
Antimicrobial agents and chemotherapy, 44(7), 1818-1824 (2000-06-20)
The penetration of two antibiotics, ampicillin and ciprofloxacin, through biofilms developed in an in vitro model system was investigated. The susceptibilities of biofilms and corresponding freely suspended bacteria to killing by the antibiotics were also measured. Biofilms of Klebsiella pneumoniae
Kenneth H Rand et al.
The Journal of antimicrobial chemotherapy, 53(3), 530-532 (2004-02-14)
We used a novel screening method to look for synergy between daptomycin and 18 other antibiotics against 19 strains of high-level vancomycin-resistant enterococci (VRE) (vancomycin MIC > or = 256 mg/L). In this approach, daptomycin was incorporated into Ca(2+)-supplemented Mueller-Hinton
Indrikis A Krams et al.
The Journal of experimental biology, 220(Pt 22), 4204-4212 (2017-09-25)
Communities of symbiotic microorganisms that colonize the gastrointestinal tract play an important role in food digestion and protection against opportunistic microbes. Diet diversity increases the number of symbionts in the intestines, a benefit that is considered to impose no cost
Carlos Pinzon-Arteaga et al.
Scientific reports, 10(1), 7411-7411 (2020-05-06)
Phenotypic selection during animal domestication has resulted in unwanted incorporation of deleterious mutations. In horses, the autosomal recessive condition known as Glycogen Branching Enzyme Deficiency (GBED) is the result of one of these deleterious mutations (102C > A), in the first exon

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service