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A5384

Sigma-Aldrich

Aconitase from porcine heart

Synonym(s):
Aconitate hydratase, Citrate (isocitrate) hydrolyase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

biological source

Porcine heart

Quality Level

form

solid

mol wt

~66 kDa by gel filtration

storage temp.

−20°C

Application

Aconitase may be usefull in diabetes research as well as to study structural conservation between iron-responsive element binding proteins (IRE-BP) and isomerases .

Packaging

150, 250 mg in serum bottle

Biochem/physiol Actions

Aconitase catalyses the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle. Aconitase is inhibited by cyanide, sulfide and copper and mercury at low concentrations. It is competitively inhibited by trans-aconitate. Aconitase contains an internal Fe-S cluster which is responsible for its activity.

Unit Definition

One unit will convert 1.0 μmole of citrate (via cis-aconitate) to isocitrate per min at pH 7.4 at 25 °C.

Analysis Note

Crude preparation containing aconitase activity

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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