Xylene Substitute

greener alternative
EC Number:

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.


hematology: suitable
histology: suitable


0.723 g/cm3

storage temp.



Non-toxic xylene replacement. Compatible with all tissue processors. May not be compatible with all permanent mounting medias.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of formalin for tissue processing. For more information see www.sigmaaldrich.com/catalog/papers/19608083.


FlameHealth hazard

Signal Word


Hazard Statements

Precautionary Statements


UN1268 - class 3 - PG 2 - VP > 110kPa - Petroleum distillates, n.o.s., HI: no

WGK Germany


Flash Point(F)

63.0 °F - closed cup

Flash Point(C)

17.2 °C - closed cup

Certificate of Analysis
Certificate of Origin
René J Buesa et al.
Annals of diagnostic pathology, 13(4), 246-256 (2009-07-18)
After the hazardous effects of xylene became indisputable in the 1970s, many potential substitutes became available, some with as many if not more hazards. This article discusses the inadequacy of 5 vegetable oils as substitutes, as well as the characteristics...
Jiaming Guo et al.
Biomedical optics express, 11(1), 99-108 (2020-02-06)
Immunohistochemical techniques, such as immunofluorescence (IF) staining, enable microscopic imaging of local protein expression within tissue samples. Molecular profiling enabled by IF is critical to understanding pathogenesis and is often involved in complex diagnoses. A recent innovation, known as microscopy...
Jiaming Guo et al.
Scientific reports, 9(1), 14578-14578 (2019-10-12)
Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that are difficult to explore using two-dimensional histology. While three-dimensional techniques...
Kyle Jablonski et al.
PloS one, 15(10), e0237520-e0237520 (2020-10-02)
Gout is the most prevalent inflammatory arthritis. To study the effects of regular physical activity and exercise intensity on inflammation and clinical outcome, we examined inflammatory pathogenesis in an acute model of murine gout and analyzed human gout patient clinical...
Alexandre Ho-Pun-Cheung et al.
British journal of cancer, 122(3), 397-404 (2019-12-04)
Triple-negative breast cancer (TNBC) has a worse prognosis compared with other breast cancer subtypes, and biomarkers to identify patients at high risk of recurrence are needed. Here, we investigated the expression of human epidermal receptor (HER) family members in TNBC...

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