A5611

Sigma-Aldrich

Bovine Serum Albumin

Cohn Fraction V, lyophilized powder, ≥96% (agarose gel electrophoresis)

Synonym(s):
Albumin bovine serum, Bovine albumin, BSA
CAS Number:
EC Number:
MDL number:
NACRES:
NA.25

Quality Level

biological source

bovine

assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

~66 kDa

packaging

poly bottle of

application(s)

immunohistochemistry: suitable

pH

4.8-7.5

solubility

H2O: soluble 40 mg/mL, clear to slightly hazy, yellow

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Looking for similar products? Visit Product Comparison Guide

General description

Serum albumins are soluble constituents of the circulatory system. Bovine serum albumin (BSA) is homologous to human serum albumin (HSA). BSA consists of three homologous domains (I, II, III), that are separated into nine loops by disulfide bonds. Serum albumin acts as a depot protein.

Application

Bovine Serum Albumin has been used:
  • for in vitro high molecular weight (HMW)-species formation assay
  • for immunohistochemistry
  • in Escherichia coli quantitative polymerase chain reaction (qPCR) analysis

Packaging

1, 5, 10 g in poly bottle

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

This is a cold alcohol fractionated albumin that is produced by Sigma-Aldrich with a stricter adherence to the original Cohn method of fractionation than other commercially available bovine albumins.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
S Valera et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(20), 9949-9953 (1992-10-15)
The major brain nicotinic acetylcholine receptor is assembled from two subunits termed alpha 4 and n alpha 1. When expressed in Xenopus oocytes, these subunits reconstitute a functional acetylcholine receptor that is inhibited by progesterone levels similar to those found...
Formation of high molecular weight p62 by CORM-3
Aki T, et al.
Testing, 14(1), e0210474-e0210474 (2019)
Standardized data quality acceptance criteria for a rapid Escherichia coli qPCR method (Draft Method C) for water quality monitoring at recreational beaches
Sivaganesan M, et al.
Water Research (2019)
X Sui et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(7), 2859-2863 (1995-03-28)
gp130, a signal-transducing receptor component of interleukin 6 (IL-6), associates with an IL-6 and IL-6 receptor (IL-6) complex and transduces signals. To examine the role of gp130 signaling in the expansion of human hemopoietic progenitor cells, we tested the effects...
Interaction of flavonoids with bovine serum albumin: a fluorescence quenching study
Papadopoulou A, et al.
Journal of Agricultural and Food Chemistry, 53(1), 158-163 (2005)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.