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A5754

Sigma-Aldrich

3-Amino-9-ethylcarbazole

≥95% (HPLC), powder

Synonym(s):
9-Ethylcarbazol-3-amine, AEC
Empirical Formula (Hill Notation):
C14H14N2
CAS Number:
Molecular Weight:
210.27
Beilstein:
174969
MDL number:
PubChem Substance ID:
NACRES:
NA.83

Quality Level

assay

≥95% (HPLC)

form

powder

impurities

≤3% solvent

solubility

ethanol: acetic acid (2:1): 50 mg/mL, clear to hazy

storage temp.

2-8°C

SMILES string

CCn1c2ccccc2c3cc(N)ccc13

InChI

1S/C14H14N2/c1-2-16-13-6-4-3-5-11(13)12-9-10(15)7-8-14(12)16/h3-9H,2,15H2,1H3

InChI key

OXEUETBFKVCRNP-UHFFFAOYSA-N

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General description

3-Amino-9-ethylcarbazole serves as chromogen in immunocytochemistry and produces a red color.

Specificity

3-Amino-9-ethylcarbazole is a peroxidase substrate suitable for use in immunoblotting and immunohistochemical staining procedures. This substrate produces an insoluble end product that is red in color and can be observed visually.

Application

3-Amino-9-ethylcarbazole has been used to visualize the bound primary antibody by using a streptavidin method. It has also been used in visualizing tissue sections from tumour samples.

Packaging

10, 50, 100 g in glass bottle

Substrates

Substrate for peroxidase.

Analysis Note

Actual content given on label

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Patterns of angiogenesis in nonsmall-cell lung carcinoma
Offersen BV, et al.
Cancer, 91(8), 1500-1509 (2001)
VEGF-C and COX-2 expression in papillary thyroid cancer
Siironen P, et al.
Endocrine-Related Cancer, 13(2), 465-473 (2006)
Cristan Herbert et al.
Respirology (Carlton, Vic.), 20(8), 1206-1212 (2015-08-21)
Exacerbations of allergic asthma are often triggered by respiratory viral infections. We have previously shown that in a T-helper type 2 (Th2)-biased cytokine environment, mouse and human airway epithelial cells (AEC) exhibit increased expression of pro-inflammatory and anti-viral genes in...
G P Harvey et al.
Journal of periodontal research, 48(2), 252-261 (2012-09-18)
The presence of citrullinated proteins, and peptidylarginine deiminase types -2 (PAD-2) and -4 (PAD-4) in periodontal tissues, determine the presence of anti-cyclic citrullinated protein antibodies (anti-CCP) in gingival crevicular fluid (GCF) and compare the expression of these proteins between inflamed...
Nicolas Kluger et al.
Clinical immunology (Orlando, Fla.), 158(2), 212-220 (2015-03-26)
Gastrointestinal dysfunction is a disabling manifestation of APECED possibly related to an autoimmune intestinal aggression. We evaluated its features in a cohort of 31 Finnish patients. The most frequent manifestations were constipation (48%), diarrhea, dysphagia and retrosternal pain (45%). AADC...

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