Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Goat Anti-Human IgG (γ-chain specific)-Peroxidase antibody is specific for human IgG when tested against human IgA, IgG, IgM, Bence Jones Kappa and Lambda myeloma proteins.
purified human IgG
Goat Anti-Human IgG (γ-chain specific)-Peroxidase antibody has been used for several ELISA-based applications.
Human serum samples were analyzed for the presence of anti-hepatitis B viral surface antigens by ELISA using HRP-conjugated goat anti-human IgG gamma chain-specific as the secondary antibody.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
1 mL in glass bottle
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
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