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A6106

Sigma-Aldrich

Aprotinin

BioUltra, 3-8 TIU/mg solid, powder, ≥98% (SDS-PAGE), suitable for inhibition assay

Synonym(s):

BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)

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$172.00
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$678.00
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About This Item

Empirical Formula (Hill Notation):
C284H432N84O79S7
CAS Number:
9087-70-1
Molecular Weight:
6511.44
EC Number:
232-994-9
MDL number:
MFCD00130541
UNSPSC Code:
12352204
NACRES:
NA.77

Key Documents

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Product Name

Aprotinin from bovine lung, BioUltra, 3-8 TIU/mg solid, ≥98% (SDS-PAGE)

biological source

bovine lung

Quality Level

300

product line

BioUltra

assay

≥98% (SDS-PAGE)

form

powder

specific activity

3-8 TIU/mg solid

mol wt

~6,500

technique(s)

inhibition assay: suitable

solubility

H2O: 5 mg/mL, clear to very slightly hazy

UniProt accession no.

P00974

storage temp.

2-8°C

SMILES string

S1SCC2NC(=O)CNC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C5NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C9N(CCC9)C(=O)CNC(=O)C(NC(=O)C(NC(=O)C%11N(CCC%11)C(=O

InChI

1S/C284H432N84O79S7/c1-21-144(9)222-271(439)337-174(68-46-105-309-282(300)301)239(407)340-187(120-160-77-85-164(374)86-78-160)251(419)341-185(116-156-55-29-24-30-56-156)250(418)342-188(121-161-79-87-165(375)88-80-161)252(420)346-191(123-208(291)378)246(41

InChI key

ZPNFWUPYTFPOJU-UHFFFAOYSA-N

Gene Information

cow ... PTI(404172)

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1 of 4

This Item
A4529A1153A3428
Aprotinin from bovine lung BioUltra, 3-8 TIU/mg solid, ≥98% (SDS-PAGE)

Sigma-Aldrich

A6106

Aprotinin from bovine lung

Aprotinin from bovine lung lyophilized powder, 3-7 TIU/mg solid

Sigma-Aldrich

A4529

Aprotinin from bovine lung

Aprotinin from bovine lung lyophilized powder, 3-8 TIU/mg solid

Sigma-Aldrich

A1153

Aprotinin from bovine lung

Aprotinin from bovine lung lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell culture

Sigma-Aldrich

A3428

Aprotinin from bovine lung

assay

≥98% (SDS-PAGE)

assay

-

assay

-

assay

-

biological source

bovine lung

biological source

bovine lung

biological source

bovine lung

biological source

bovine lung

Gene Information

cow ... PTI(404172)

Gene Information

cow ... PTI(404172)

Gene Information

cow ... PTI(404172)

Gene Information

cow ... PTI(404172)

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

200

form

powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

solubility

H2O: 5 mg/mL, clear to very slightly hazy

solubility

H2O: >10 mg/mL

solubility

H2O: ≥5 mg/mL

solubility

H2O: 5 mg/mL

Application

Aprotinin is largely used as an inhibitor of trypsin.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

Preparation Note

A further purification of A1153 to yield a product with slightly higher activity and purity

Other Notes

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.
One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000477864
0000477863
0000477864
0000477863
0000476220

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Cecilia B Levandowski et al.
PLoS biology, 19(8), e3001364-e3001364 (2021-08-06)
The naturally occurring Δ40p53 isoform heterotetramerizes with wild-type p53 (WTp53) to regulate development, aging, and stress responses. How Δ40p53 alters WTp53 function remains enigmatic because their co-expression causes tetramer heterogeneity. We circumvented this issue with a well-tested strategy that expressed
Luciana Mateus Gonçalves et al.
Frontiers in endocrinology, 11, 602519-602519 (2021-02-02)
Pancreatic islets are clusters of endocrine cells that secrete different hormones to regulate blood glucose levels. Efficient hormone secretion requires a close interaction of endocrine cells with their vascular system. Islets receive blood through feeding arteriole(s) that branch into capillaries
Luming Wan et al.
Nature metabolism, 4(1), 29-43 (2022-01-08)
Severe cases of infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are associated with elevated blood glucose levels and metabolic complications. However, the molecular mechanisms for how SARS-CoV-2 infection alters glycometabolic control are incompletely understood. Here, we connect the
Benjamin J Patty et al.
Nature protocols, 16(5), 2633-2666 (2021-04-30)
Determining chromatin-associated protein localization across the genome has provided insight into the functions of DNA-binding proteins and their connections to disease. However, established protocols requiring large quantities of cell or tissue samples currently limit applications for clinical and biomedical research
Christian M Cohrs et al.
Cell reports, 31(1), 107469-107469 (2020-04-09)
Type 2 diabetes is characterized by peripheral insulin resistance and insufficient insulin release from pancreatic islet β cells. However, the role and sequence of β cell dysfunction and mass loss for reduced insulin levels in type 2 diabetes pathogenesis are
View All Related Papers

Articles

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

Protocols

Enzymatic Assay of Aprotinin

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

Questions

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