We have no data and have not studied whether catalog number A6154 is cross adsorbed against human, mouse, and bovine sera.
A6154
Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Goat Anti-Rabbit IgG (whole molecule)–HRP
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biological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rabbit
technique(s)
direct ELISA: 1:10,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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This Item | A4914 | A9169 | A8275 |
|---|---|---|---|
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate peroxidase conjugate |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form IgG fraction of antiserum | antibody form IgG fraction of antiserum |
| technique(s) direct ELISA: 1:10,000 | technique(s) direct ELISA: 1:10,000 | technique(s) direct ELISA: 1:50,000 using using 5 μg/ml of rabbit IgG for coating and OPD substrate, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400, western blot (chemiluminescent): 1:80,000-1:160,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per well) | technique(s) direct ELISA: 1:10,000 |
| species reactivity rabbit | species reactivity - | species reactivity rabbit | species reactivity - |
| biological source goat | biological source goat | biological source goat | biological source goat |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
General description
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody is immunospecific for rabbit IgG by immunoelectrophoresis against normal serum and rabbit IgG, prior to conjugation.
Immunogen
purified rabbit IgG
Application
C33A cell lysates were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Protein aliquots from colonic mucosal extracts (μg) were anlayzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody at a 1:5000 dilution in TBSt for 1 hr at room temperature.
Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody has been used for western blot (1:6,000), and ELISA assays.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.
Preparation Note
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
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Is this polyclonal cross adsorbed against human, mice, bovine sera
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What is the concentration of the antibody? Please specify in µg/mL.
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The concentration of this antibody preparation is lot specific and reported in the Certificate of Analysis. A review of recent lots indicates a typical range of 0.5 - 1.0 mg/mL. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/a6154#product-documentationHelpful?
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Per quanto tempo è possibile conservare la confezione alle condizioni specificate (flacone chiuso)?
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This product is not assigned an expiration or retest date. When stored unopened at the recommended storage temperature of -20°C, the material is quite stable. Products with no assigned expiration date are covered by a warranty of 1 year from the date of shipment. This antibody may be thawed, aliquoted, and re-frozen upon receipt. Thawed aliquots are stable for day-to-day use for up to one month at 2 - 8°C. Please see the link below for additional information provided in the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/649/246/a6154dat.pdfHelpful?
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