A6270

Azure A chloride

Dye content ≥70 %, Powder or crystals

• Synonym(s): Azure eosin methylene blue; 7-Amino-N,N-dimethyl-3H-phenothiazin-3-iminium chloride
• Empirical Formula (Hill Notation): C₁₄H₁₄ClN₃S
• CAS Number: 531-53-3
• Molecular Weight: 291.80
• Colour Index Number: 52005
• Beilstein/REAXYS Number: 3922287
• EC Number: 208-510-7
• MDL number: MFCD00012112
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891073
• NACRES: NA.47

Properties

• Product Name: Azure A chloride, Dye content ≥70 %
• form: powder or crystals
• Quality Level: 200
• composition: Dye content, ≥70%
• color: green to very dark green
• mp: 290 °C (dec.) (lit.)
• solubility: water: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]
• InChI: 1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
• InChI key: NALREUIWICQLPS-UHFFFAOYSA-N

Description

Application

Azure A chloride has been used for the staining of polyacrylamide gel to analyse the bovine lung heparin digestion by heparinase I.[1] It has been used for the determination of the sulphated groups content in polysaccharides.[2]

Biochem/physiol Actions

Azure A is a basic cationic dye.[3] The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels.[4] It is also used for the staining of chromosomes in the nucleus.[5] It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain.[3]

Caution

Handle and store under argon. Glove bag item.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)