MilliporeSigma
All Photos(3)

A6270

Sigma-Aldrich

Azure A chloride

Dye content ≥70 %

Empirical Formula (Hill Notation):
C14H14ClN3S
CAS Number:
Molecular Weight:
291.80
Colour Index Number:
52005
Beilstein:
3922287
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder or crystals

Quality Level

composition

Dye content, ≥70%

mp

290 °C (dec.) (lit.)

solubility

water: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
861049A2918200212
Azure A chloride Dye content ≥70 %

Sigma-Aldrich

A6270

Azure A chloride

Azure A chloride certified by the Biological Stain Commission, Dye content 70 %

Sigma-Aldrich

861049

Azure A chloride

Azure A chloride certified by the Biological Stain Commission

Sigma-Aldrich

A2918

Azure A chloride

Azure A eosinate

Sigma-Aldrich

200212

Azure A eosinate

composition

Dye content, ≥70%

composition

Dye content, 70%

composition

Dye content, ≥75%

composition

-

mp

290 °C (dec.) (lit.)

mp

290 °C (dec.) (lit.)

mp

290 °C (dec.) (lit.)

mp

-

solubility

water: 1 mg/mL

solubility

H2O: 1 mg/mL

solubility

water: 1 mg/mL

solubility

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Application

Azure A chloride has been used for the staining of polyacrylamide gel to analyse the bovine lung heparin digestion by heparinase I. It has been used for the determination of the sulphated groups content in polysaccharides.

Biochem/physiol Actions

Azure A is a basic cationic dye. The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain.

Caution

Handle and store under argon. Glove bag item.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 7

1 of 7

Seratrodast ≥98% (HPLC)

Sigma-Aldrich

SML1379

Seratrodast

THIONINE BLUE GO AldrichCPR

R310360

THIONINE BLUE GO

Thionin acetate salt certified by the Biological Stain Commission, Dye content ≥85 %

Sigma-Aldrich

861340

Thionin acetate salt

Pyronin B Dye content ≥30 %

Sigma-Aldrich

P6892

Pyronin B

Toluidine Blue O Technical grade

Sigma-Aldrich

T3260

Toluidine Blue O

Biochemical and antiviral activities of enzymatic hydrolysates from different invasive French seaweeds.
Hardouin K, et al.
Journal of Applied Phycology, 26.2, 1029-1042 (2014)
How Romanowsky stains work and why they remain valuable - including a proposed universal Romanowsky staining mechanism and a rational troubleshooting scheme.
Horobin RW
Biotechnic & Histochemistry, 86, 36-51 (2011)
Generating heparan sulfate saccharide libraries for glycomics applications.
Powell AK, et al.
Nature Protocols, 5, 821-833 (2010)
Quantification and characterization of glycosaminoglycans at the nanogram level by a combined azure A-silver staining in agarose gels.
van de Lest CH, et al.
Analytical Biochemistry, 221, 356-361 (1994)
P Chieco et al.
The Histochemical journal, 25(8), 569-577 (1993-08-01)
Mitotic index is a clinically important parameter in cancer pathology. We developed a staining method using Toluidine Blue to detect efficiently and rapidly mitotic figures in sections of formalin-fixed paraffin-embedded human and rat tissues. Sections were stained at acid pH

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service