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A6270

Sigma-Aldrich

Azure A chloride

Dye content ≥70 %

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Empirical Formula (Hill Notation):
C14H14ClN3S
CAS Number:
531-53-3
Molecular Weight:
291.80
Colour Index Number:
52005
Beilstein:
3922287
EC Number:
208-510-7
MDL number:
MFCD00012112
PubChem Substance ID:
24891073
NACRES:
NA.47

form

powder or crystals

Quality Level

200

composition

Dye content, ≥70%

mp

290 °C (dec.) (lit.)

solubility

water: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

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This Item
861049A2918200212
Azure A chloride Dye content ≥70 %

Sigma-Aldrich

A6270

Azure A chloride

Azure A chloride certified by the Biological Stain Commission, Dye content 70 %

Sigma-Aldrich

861049

Azure A chloride

Azure A chloride certified by the Biological Stain Commission

Sigma-Aldrich

A2918

Azure A chloride

Azure A eosinate

Sigma-Aldrich

200212

Azure A eosinate

composition

Dye content, ≥70%

composition

Dye content, 70%

composition

Dye content, ≥75%

composition

-

mp

290 °C (dec.) (lit.)

mp

290 °C (dec.) (lit.)

mp

290 °C (dec.) (lit.)

mp

-

solubility

water: 1 mg/mL

solubility

H2O: 1 mg/mL

solubility

water: 1 mg/mL

solubility

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Application

Azure A chloride has been used for the staining of polyacrylamide gel to analyse the bovine lung heparin digestion by heparinase I. It has been used for the determination of the sulphated groups content in polysaccharides.

Biochem/physiol Actions

Azure A is a basic cationic dye. The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain.

Caution

Handle and store under argon. Glove bag item.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Biochemical and antiviral activities of enzymatic hydrolysates from different invasive French seaweeds.
Hardouin K, et al.
Journal of Applied Phycology, 26.2, 1029-1042 (2014)
How Romanowsky stains work and why they remain valuable - including a proposed universal Romanowsky staining mechanism and a rational troubleshooting scheme.
Horobin RW
Biotechnic & Histochemistry, 86, 36-51 (2011)
Generating heparan sulfate saccharide libraries for glycomics applications.
Powell AK, et al.
Nature Protocols, 5, 821-833 (2010)
Quantification and characterization of glycosaminoglycans at the nanogram level by a combined azure A-silver staining in agarose gels.
van de Lest CH, et al.
Analytical Biochemistry, 221, 356-361 (1994)
P Chieco et al.
The Histochemical journal, 25(8), 569-577 (1993-08-01)
Mitotic index is a clinically important parameter in cancer pathology. We developed a staining method using Toluidine Blue to detect efficiently and rapidly mitotic figures in sections of formalin-fixed paraffin-embedded human and rat tissues. Sections were stained at acid pH
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