A6306

Sigma-Aldrich

Agarase from Pseudomonas atlantica

lyophilized powder, ≥5,000 units/mg protein (Lowry)

Synonym(s):
Agarose 4-glycanohydrolase, β-Agarase
CAS Number:
Enzyme Commission number:
MDL number:
eCl@ss:
42040102
NACRES:
NA.26

form

lyophilized powder

Quality Level

specific activity

≥5,000 units/mg protein (Lowry)

storage temp.

2-8°C

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Related Categories

Packaging

1000 units in serum bottle

Unit Definition

One unit will produce 1.0 μg of reducing sugar (measured as D-galactose) from agar per min at pH 6.0 at 40 °C.

Physical form

Contains phosphate buffer salts. May contain bovine serum albumin to standardize protein content

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Effects of temperature, light level, photoperiod, and ammonium concentration on Pyropia leucosticta (Bangiales, Rhodophyta) from the Northwest Atlantic
Lindsay Gree
Journal of Applied Phycology (2015)
Seungwoo Lee et al.
Journal of microbiology and biotechnology, 21(11), 1116-1122 (2011-12-01)
In this study, site-directed mutagenesis was performed on the β-agarase AgaA gene from Zobellia galactanivorans to improve its catalytic activity and thermostability. The activities of three mutant enzymes, S63K, C253I, and S63K-C253I, were 126% (1,757.78 U/mg), 2.4% (33.47 U/mg), and...
?-Agarases I and II from Pseudomonas atlantica
Lora M
FEBS Journal (1983)
Young Joon Rhee et al.
Journal of microbiology and biotechnology, 20(10), 1378-1385 (2010-10-30)
A novel agarolytic bacterium KY-YJ-3, producing extracellular agarase, was isolated from the freshwater sediment of the Sincheon River in Daegu, Korea. On the basis of gram-staining data, morphology, and phylogenetic analysis of the 16S rDNA sequence, the isolate was identified...
Yuji Hatada et al.
Marine biotechnology (New York, N.Y.), 13(3), 411-422 (2010-08-06)
A gene of unknown function from the genome of the agar-degrading deep-sea bacterium Microbulbifer thermotolerans JAMB-A94(T) was functionally identified as a ι-carrageenase gene. This gene, designated as cgiA, is located together with two β-agarase genes, agaA and agaO in a...

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