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A6535

Apyrase from potatoes

ATPase ≥200 units/mg protein, lyophilized powder

Synonym(s):

Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

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100 UNITS

$161.00

200 UNITS

$302.00

500 UNITS

$538.00

1000 UNITS

$833.00

2000 UNITS

$1,500.00

$161.00

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About This Item

CAS Number:
9000-95-7
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-569-8
MDL number:
MFCD00130542
EC Number:
3.6.1.5 (BRENDA, IUBMB)

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Product Name

Apyrase from potatoes, ATPase ≥200 units/mg protein, lyophilized powder

type

Grade VII

form

lyophilized powder

ATPase activity

≥200 units/mg protein

secondary activity

≥50 % of base activity ADPase

composition

Protein, 25-60%

solubility

H2O: soluble 1.0 mg/mL

application(s)

diagnostic assay manufacturing

foreign activity

Acid Phosphatase ≤2% of base activity

storage temp.

−20°C

Quality Level

300

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This Item
SRE0019A7646A6410
Apyrase from potatoes ATPase ≥200 units/mg protein, lyophilized powder

Sigma-Aldrich

A6535

Apyrase from potatoes

Sigma-Aldrich

Sigma-Aldrich

SRE0019

Apyrase from potatoes

Apyrase from potatoes ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

Sigma-Aldrich

A7646

Apyrase from potatoes

Apyrase from potatoes ATPase ≥200 units/mg protein, lyophilized powder

Sigma-Aldrich

A6410

Apyrase from potatoes

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder (partially purified)

form

lyophilized powder

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

solubility

H2O: soluble 1.0 mg/mL

solubility

H2O: soluble 1.0 mg/mL

solubility

H2O: soluble 10 mg/mL, slightly hazy

solubility

H2O: soluble 1.0 mg/mL

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

foreign activity

Acid Phosphatase ≤2% of base activity

foreign activity

Acid Phosphatase ≤2% of base activity

foreign activity

Acid Phosphatase ≤5% of base activity

foreign activity

Acid Phosphatase ≤2% of base activity

Quality Level

300

Quality Level

400

Quality Level

200

Quality Level

200

Application

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1.[1] Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation [2].
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis [3]. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Other Notes

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
This grade has a low ATPase/ADPase ratio.

Packaging

Sold on the basis of ATPase units.

Physical form

Partially purified, lyophilized powder containing potassium succinate buffer salts.

Preparation Note

Derived from red potato

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000477967
0000477967
0000476378
0000476378
0000476391

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Serena Sirigu et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(47), E7448-E7455 (2016-11-07)
Direct inhibition of smooth muscle myosin (SMM) is a potential means to treat hypercontractile smooth muscle diseases. The selective inhibitor CK-2018571 prevents strong binding to actin and promotes muscle relaxation in vitro and in vivo. The crystal structure of the
Yiying Cai et al.
Microorganisms, 8(10) (2020-10-02)
Traditional in vitro time-kill studies (TKSs) require viable plating, which is tedious and time-consuming. We used ATP bioluminescence, with the removal of extracellular ATP (EC-ATP), as a surrogate for viable plating in TKSs against carbapenem-resistant Gram-negative bacteria (CR-GNB). Twenty-four-hour TKSs
Alexandra L Chang-Graham et al.
Science (New York, N.Y.), 370(6519) (2020-11-21)
Rotavirus causes severe diarrheal disease in children by broadly dysregulating intestinal homeostasis. However, the underlying mechanism(s) of rotavirus-induced dysregulation remains unclear. We found that rotavirus-infected cells produce paracrine signals that manifested as intercellular calcium waves (ICWs), observed in cell lines
Properties of Two Apyrases from Solanum tuberosum
Kettlun, A. et al.
Phytochemistry, 21(3), 551-558 (1982)
Agnete Kirkeby et al.
Thrombosis and haemostasis, 99(4), 720-728 (2008-04-09)
The haematopoietic hormone erythropoietin (EPO) has neuroprotective properties and is currently being explored for treatment of stroke and other neurological disorders. Short-term, high-dose treatment with EPO seems to improve neurological function of stroke patients but may be associated with increased
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