A6778

Sigma-Aldrich

Angiotensin Converting Enzyme from rabbit lung

≥2.0 units/mg protein (modified Warburg-Christian)

Synonym(s):
Peptidyldipeptide Hydrolase, ACE, Peptidyl-dipeptidase A
CAS Number:
Enzyme Commission number:
MDL number:
eCl@ss:
42010109
NACRES:
NA.32

form

lyophilized powder

Quality Level

specific activity

≥2.0 units/mg protein (modified Warburg-Christian)

shipped in

dry ice

storage temp.

−20°C

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General description

The angiotensin-converting enzyme (ACE) is a dipeptidyl-carboxypeptidase which exists in somatic and testicular isoforms with zinc binding motif HEXXH in their active site. ACE regulates blood pressure through renin-angiotensin system. ACE elevates blood pressure by converting angiotensin I to a key vasoconstrictor angiotensin II and inhibiting a potent vasodilator bradykinin. Inhibition of ACE is a targeted therapeutic strategy for high blood pressure. Several ACE synthetic inhibitory peptides available for clinical use include captopril, enalapril and lisinopril. Currently, developing inhibitory peptides from natural food sources, or phenolic compounds from plant sources to inhibit ACE is underway. ACE plays a critical role in fertilization by releasing the proteins anchored to glycosylphosphatidylinositol (GPI) in sperm membrane.

Application

Angiotensin converting enzyme from rabbit lung has been used:
  • for measuring inhibitory effect of egg white protein hydrolysates on ACE activity by high performance liquid chromatography (HPLC)
  • to measure the ACE inhibition by litchi pericarp and cooked chicken breast using hippuryl-L-histidyl-L-leucine (HHL) as substrate by reverse phase-HPLC (RP-HPLC)3 and HPLC respectively
  • in releasing GPI anchored protein in vitro in few cell lines like HeLa, HEK293 and in vivo in mice sperm.

Biochem/physiol Actions

Removes C-terminal dipeptides from susceptible substrates, e.g., angiotensin I and bradykinin.

Quality

May contain traces of sodium chloride.

Unit Definition

One unit will produce 1.0 μmole of hippuric acid from Hippuryl-His-Leu per min in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 °C.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Are there any other known inhibitors of rabbit lung Angiotensin Converting Enzyme (ACE), Product A6778?

    Idrapril, the prototype of a new class of angiotensin converting enzyme (ACE) inhibitors, competitively inhibits, with nanomolar apparent Ki, the hydrolysis of hippuryl-glycyl-glycine by rabbit lung ACE.  Kinetic Ki and kon and koff values were 470 pM, 3.0±1.5×106 M-1sec-1 and 1.4±0.3×10-3 sec-1, respectively.  Reference: Biochemical Pharmacology, 45(6), 1358-1362 (1993).

  6. What is the medical significance of Angiotensin Converting Enzyme (ACE), Product A6778?

    Angiotensin-converting enzyme (ACE) produces angiotensin II, a substance that affects the cardiovascular system in numerous ways, including narrowing of blood vessels. This narrowing can cause high blood pressure and cause the heart to work harder.  ACE inhibitors treat a variety of conditions, such as high blood pressure, scleroderma and migraines. (Mayo Clinic)

  7. What is the effect of bleomycin on Angiotensin Converting Enzyme (ACE) activity in vivo?

    Bleomycin causes a marked reduction in rabbit lung ACE activity. Reference: Cancer Research, 40, 3621-3626, (1980).

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Angiotensin-converting enzyme inhibitory activity of milk protein hydrolysates: Effect of substrate, enzyme and time of hydrolysis
Otte J, et al.
International dairy journal, 17(5), 488-503 (2007)
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Diminished release and function of endothelium-derived nitric oxide coupled with increases in reactive oxygen species production is critical in endothelial dysfunction. Recent evidences have shown that activation of the protective axis of the renin-angiotensin system composed by angiotensin-converting enzyme 2...
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The Journal of clinical investigation, 123(12), 5203-5211 (2013-11-02)
Angiotensin converting enzyme 2 (ACE2) is a negative regulator of the renin-angiotensin system (RAS), catalyzing the conversion of Angiotensin II to Angiotensin 1-7. Apelin is a second catalytic substrate for ACE2 and functions as an inotropic and cardioprotective peptide. While...

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