A6964
sterile-filtered
liquid
400-600 units/mL
cell culture | mammalian: suitable
6.8-7.8
dry ice
−20°C
10 - Combustible liquids
WGK 2
Not applicable
Not applicable
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It can be used for detaching cells, analysis of cell surface markers and virus growth assays.
This product is not tested for tissue, but it should work on soft tissue.
This product is typically at a concentration of 400-600 units/mL.
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Accutase is purified from a marine invertebrate. There are no other animal or bacterial derived proteins present.
Accutase is a mixture of non-mammalian proteases. Accumax is accutase that has been augmented with DNase to reduce clumping associated with DNA release from damaged cells.
Accutase®, product A6964, or Accumax™ (Accutase® with DNase), product A7089, will work well for dissociation of ES cells. The Accutase® product has protease and dispase activity to dissociate the cells. The Accumax™ product also has DNase to limit the aggregation that is caused by DNA released from ruptured cells during the dissociation/scraping.Please see the reference and link below for more information on the use of Accutase® with ES cells:Mol Reprod Dev. 2008 May;75(5):818-27
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Due to its gentle nature Accutase® cell detachment solution usually does not need to be neutralized following cell passaging. Accutase® is also heat inactivated after 45 minutes at 37°C. If this method of neutralization does not work for your cells, or if you want to neutralize Accutase® in a shorter period of time, the addition of 10% FBS containing media will accomplish this task. Experiments have shown that diluting a sample of Accutase® with 10% with fetal bovine serum achieved an environment similar to that found in cell culture. Measurements of the enzyme activity in this diluted sample showed decreased activity over time, falling below that of the required level for cell detachment (>175 units/mL). After about 6 minutes of serum interaction, the enzyme activity of Accutase® was well below this level, demonstrating effective inhibition by FBS.
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