A6970

Sigma-Aldrich

Monoclonal Anti-Acetylcholinesterase antibody produced in mouse

clone ZR3, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Monoclonal Anti-AChE
MDL number:
Pricing and availability is not currently available.

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ZR3, monoclonal

form

buffered aqueous solution

species reactivity

rat, guinea pig, human, rabbit, mouse, feline

application(s)

flow cytometry: 3-5 μg
immunofluorescence: 1:20
immunohistochemistry: 1:20
immunoprecipitation (IP): suitable
western blot: 1:500-1:1000

isotype

IgG2b

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

rat ... Ache(83817)

Immunogen

Purified rat brain acetylcholinesterase.

Biochem/physiol Actions

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Physical form

Solution in 0.01 M phosphate buffered saline containing 1 mg/mL BSA and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Z Rakonczay et al.
Journal of neurochemistry, 46(1), 280-287 (1986-01-01)
Seven unique monoclonal antibodies were generated to rat brain acetylcholinesterase. Upon density gradient ultracentrifugation, immunoglobulin complexes with the monomeric enzyme appeared as single peaks of acetylcholinesterase activity with a sedimentation coefficient approximately 3S greater than that of the free enzyme....
Jose R Suarez-Lopez et al.
Pediatrics, 132(6), e1649-e1658 (2013-11-20)
Organophosphate exposures can affect children's neurodevelopment, possibly due to neurotoxicity induced by acetylcholinesterase (AChE) inhibition, and may affect boys more than girls. We tested the hypothesis that lower AChE activity is associated with lower neurobehavioral development among children living in...
Miroslav Pohanka
Expert opinion on therapeutic patents, 22(8), 871-886 (2012-07-10)
Both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are present in the body in large amounts. AChE is an important part of the cholinergic nervous system taking place in the central and peripheral nervous system. AChE is a target of several toxins...
Holmes, C. et al.
The Journal of Neuroscience, 49, 207-207 (1997)
Matthew W Rice et al.
Frontiers in neuroanatomy, 5, 53-53 (2011-09-03)
The striatum is a major component of the basal ganglia and is associated with motor and cognitive functions. Striatal pathologies have been linked to several disorders, including Huntington's, Tourette's syndrome, obsessive-compulsive disorders, and schizophrenia. For the study of these striatal...

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