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A7030

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%

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Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

biological source

bovine

Quality Level

assay

≥98%

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

origin

USA origin

free fatty acid content

≤0.02%

technique(s)

ELISA: suitable
blood typing: suitable
ligand binding assay: suitable

impurities

essentially globulin free

loss

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

foreign activity

protease ≤0.005 U/mg

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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1 of 4

This Item
A3059A0281A3803
vibrant-m

A7030

Bovine Serum Albumin

vibrant-m

A3059

Bovine Serum Albumin

vibrant-m

A0281

Bovine Serum Albumin

vibrant-m

A3803

Bovine Serum Albumin

technique(s)

ELISA: suitable, ligand binding assay: suitable, blood typing: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

enzyme immunoassay: suitable

technique(s)

cell culture | mammalian: suitable

assay

≥98%

assay

≥98%

assay

≥99% (agarose gel electrophoresis)

assay

≥98% (agarose gel electrophoresis)

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

General description

Bovine serum albumin is a protein which has a key role in regulating blood colloidal osmotic pressure. A water-soluble protein composed of 583 amino acids, BSA has a calculated molecular weight of 66,430 Daltons. Six α-helices form three homologous domains of BSA. Depending on pH, BSA undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

This BSA product is specifically evaluated and tested for the following properties:
  • Very low protease content / protease-free
  • Very low fatty acid content / fatty acid-free

Protease-free BSA is especially useful for applications like:
  • Enzyme assays
  • Protein-based assays
  • Protease-sensitive techniques that include:
a. Enzyme immunoassay (EIA)
b. Nucleic acid hybridization
c. Radioimmunoassay (RIA)

Application

Bovine Serum Albumin has been used:
  • as a constituent of medium used for the differentiation of human pluripotent stem cells
  • in enzyme-linked immunosorbent assay (ELISA) and sandwich ELISA
  • to permeabilize fetal cortical cells

This A7030 BSA has specifically been used as a component of blocking buffer in a peptide-based ELISA assay for analysis of COVID-19 / SARS-CoV-2 samples (Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020)).

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Heat shock fractionated
  • Protease-free
  • Fatty acid-free
  • Essentially globulin-free

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

also commonly purchased with this product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Bovine Serum Albumin

Xueying Zhao et al.
International journal of molecular sciences, 20(14) (2019-08-03)
Diabetic nephropathy is increasingly recognized as a major contributor to kidney failure in patients with obesity and type 2 diabetes. This study was designed to identify the molecular mediators of kidney injury associated with metabolic syndrome with or without hyperglycemia.
Hiroyuki Shinto et al.
Colloids and surfaces. B, Biointerfaces, 181, 270-277 (2019-06-04)
It is very important to examine carefully the potential adverse effects of engineered nanoparticles (NPs) on human health and environments. In the present study, we have investigated the impact of interfacial serum proteins on the cell membrane disruption induced by
Human pluripotent stem cell-derived acinar/ductal organoids generate human pancreas upon orthotopic transplantation and allow disease modelling.
Hohwieler M
Gut, 66(3), 473-486 (2017)
Fixed single-cell transcriptomic characterization of human radial glial diversity
Elliot R Thomsen
Nature Methods, 13(1), 87-93 (2016)
Phosphorylated fraction of H2AX as a measurement for DNA damage in cancer cells and potential applications of a novel assay
Jiuping Ji
PLoS ONE (2017)

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