This product is not routinely tested for Biotin. However, this material is dialyzed with a 10 kDa cutoff membrane. This dialysis step removes small molecules such as biotin, which has a molecular weight of less than 500 Da. Therefore, this product is considered low in biotin, helping to prevent interference with streptavidin–biotin binding.
A7030
Bovine Serum Albumin
heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%
Bovine Serum Albumin
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 g | Available to ship TODAYfromMILWAUKEE | $167.00 | |
| 50 g | Available to ship TODAYfromMILWAUKEE | $526.00 | |
| 100 g | Available to ship TODAYfromMILWAUKEE | $791.00 | |
| 500 g | Available to ship TODAYfromMILWAUKEE | $3,170.00 | |
| 1 kg | Available to ship TODAYfromMILWAUKEE | $4,950.00 | |
| 5 kg | Available to ship TODAYfromMILWAUKEE | $18,680.00 |
About This Item
CAS Number:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
ELISA: suitable, blood typing: suitable, ligand binding assay: suitable
Impurities:
essentially globulin free
Concentration:
≥95% protein
biological source
bovine
Quality Level
assay
≥98%
form
lyophilized powder
purified by
heat shock fractionation
origin
USA origin
concentration
≥95% protein
free fatty acid content
≤0.02%
technique(s)
ELISA: suitable, blood typing: suitable, ligand binding assay: suitable
impurities
essentially globulin free
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
protease ≤0.005 U/mg
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
General description
Bovine serum albumin is a protein which has a key role in regulating blood colloidal osmotic pressure. A water-soluble protein composed of 583 amino acids, BSA has a calculated molecular weight of 66,430 Daltons. Six α-helices form three homologous domains of BSA. Depending on pH, BSA undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.
This BSA product is specifically evaluated and tested for the following properties:
Protease-free BSA is especially useful for applications like:
b. Nucleic acid hybridization
c. Radioimmunoassay (RIA)
This BSA product is specifically evaluated and tested for the following properties:
- Very low protease content / protease-free
- Very low fatty acid content / fatty acid-free
Protease-free BSA is especially useful for applications like:
- Enzyme assays
- Protein-based assays
- Protease-sensitive techniques that include:
b. Nucleic acid hybridization
c. Radioimmunoassay (RIA)
Application
Bovine Serum Albumin has been used:
This A7030 BSA has specifically been used as a component of blocking buffer in a peptide-based ELISA assay for analysis of COVID-19 / SARS-CoV-2 samples (Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020)).
- as a constituent of medium used for the differentiation of human pluripotent stem cells
- in enzyme-linked immunosorbent assay (ELISA) and sandwich ELISA
- to permeabilize fetal cortical cells
This A7030 BSA has specifically been used as a component of blocking buffer in a peptide-based ELISA assay for analysis of COVID-19 / SARS-CoV-2 samples (Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020)).
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Features and Benefits
- Heat shock fractionated
- Protease-free
- Fatty acid-free
- Essentially globulin-free
Preparation Note
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
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This Item | |||
|---|---|---|---|
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| technique(s) ELISA: suitable, ligand binding assay: suitable, blood typing: suitable | technique(s) ELISA: suitable, western blot: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| assay ≥98% | assay ≥98% | assay ≥98% | assay ≥98% (agarose gel electrophoresis) |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| A7030-100G | 04061835553563 |
| A7030-5G | 04061833425954 |
| A7030-1KG | 04061835553587 |
| A7030-100UG-KC | 04061833463338 |
| A7030-500G | 04061835553594 |
| A7030-50G | 04061835553600 |
| A7030-10G | 04061835553570 |
| A7030-5KG | 04061835540617 |
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Is this product biotin-free?
1 answer-
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If this material was inadvertently stored at room temperature, what is the room temperature stability?
1 answer-
This product is shipped at ambient temperature and is stable at ambient temperature for up to two weeks. For long term storage refrigerated temperature is recommended.
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How long would a 20% BSA solution be stable in water stored at 4C?
1 answer-
If stored at 2-8 °C, a filter sterilized 20% BSA solution in water or aqueous buffer is generally stable for a minimum of 2.5 - 3 years.
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Is it possible that BSA contains ions such as Mg2+? I am considering adding it to the buffer, but I am concerned that it might activate the enzyme.
1 answer-
The magnesium content of this material is not routinely tested, but historical data indicates that three lots contained between 10-20 parts per million. Water is used as the pre-lyophilization solvent.
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Is product number A7030, Bovine Serum Albumin (BSA) glycosylated?
1 answer-
Unfortunately, there is currently no information available regarding the glycosylation status of BSA product A7030.
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How does protease content determined? With this value all proteases are included? Or only refers to plasmatic proteases.
1 answer-
The specific method used to test this material for protease activity is considered proprietary. However, it is based on the enzymatic cleavage of FITC-Casein. See the link below to review the FITC-Casein Enzymatic Assay to demonstrate the principal of this method.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/353/850/c3777enz.pdfHelpful?
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what is the expiry of this product?
1 answer-
This product is not assigned an expiration date. It is assigned a recommended retest date of 5 years after the quality release date. The recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
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What is the extinction coefficient of this product?
1 answer-
The extinction coefficient for item A7030 has not been determined.
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is it suitable for cell culture?
1 answer-
This product has not been tested for suitability in cell culture. The end user would have to determine suitability. Please see the link below for a cell culture tested version of heat shock fractionated BSA:
https://www.sigmaaldrich.com/product/sigma/a4161Helpful?
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My BSA product is not sterile (open outside BSC hood)--- Can I filter-sterilize with 0.2um filter to use with viable cells solution?
1 answer-
Yes, prepared solutions of bovine serum albumin may be filter sterilized for use in cell culture by using a 0.22 um filter membrane.
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