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A7080

Sigma-Aldrich

Amphiregulin human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

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Synonym(s):
AREG, CRDGF, SDGF, AR
CAS Number:
117147-70-3
MDL number:
MFCD00282118
NACRES:
NA.32

biological source

human

Quality Level

100

recombinant

expressed in E. coli

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

10-100 ng/mL ED50/EC50

mol wt

~11 kDa

packaging

pkg of 1 mg

storage condition

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P15514

storage temp.

−20°C

Gene Information

human ... AREG(374)

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This Item
SRP6201A0476E1388
Amphiregulin human recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

Sigma-Aldrich

A7080

Amphiregulin human

Amphiregulin human recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

Sigma-Aldrich

SRP6201

Amphiregulin human

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Sigma-Aldrich

A0476

Activin Receptor-Like Kinase 1/Fc Chimera human

Sigma-Aldrich

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E1388

β-Endothelial Cell Growth Factor human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in NSO cells

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

>90% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

potency

10-100 ng/mL ED50/EC50

potency

-

potency

-

potency

0.03-0.3 ng/mL ED50/EC50

mol wt

~11 kDa

mol wt

11.3 kDa

mol wt

50-55 kDa by SDS-PAGE (reducing), calculated mol wt ~37.3 kDa

mol wt

predicted mol wt ~17 kDa

Biochem/physiol Actions

Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.

Components

Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.

Caution

This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.

Preparation Note

It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.

Analysis Note

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is typically 5-15 ng/mL.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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