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MilliporeSigma

A7095

Sigma-Aldrich

Amyloglucosidase from Aspergillus niger

≥260 U/mL, aqueous solution

Synonym(s):

AMG 300L, 1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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50 ML
$142.00

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50 ML
$142.00

About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

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$142.00


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biological source

Aspergillus niger

form

aqueous solution

specific activity

≥260 U/mL

density

~1.2 g/mL at 25 °C

storage temp.

2-8°C

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1 of 4

This Item
10113A2910A9913
specific activity

≥260 U/mL

specific activity

~120 U/mg

specific activity

-

specific activity

-

biological source

Aspergillus niger

biological source

-

biological source

-

biological source

-

form

aqueous solution

form

powder

form

powder

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

density

~1.2 g/mL at 25 °C

density

-

density

-

density

-

General description

Stabilized with glucose.
Amyloglucosidase is a disaccharidase−type alpha-glucosidase,[1] produced by several species of Aspergillus genus.[2] Immobilization of amyloglucosidase is known to increased its stability.[3]

Application

Amyloglucosidase from Aspergillus niger has been used in in vitro digestions.[4] It has also been used in the isolation of insoluble and soluble dietary fibre from quinoa and amaranth.[5]

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides.[6] Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry for the commercial production of D-glucose from corn syrups.[7][8]

Legal Information

A product of Novozymes Corp.
AMG is a trademark of Novozymes Corp.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Questions

1–3 of 3 Questions  
  1. Can I dilute the amyloglucosidase enzyme (A7095) in distilled/deionized water or should I use any specific buffer for dilution?

    1 answer
    1. Unfortunately, the formulation of this solution is considered proprietary. There is no information regarding further dilutions, however the optimum pH is 4.5. Any aqueous diluent should be in this approximate range. See the table below to review the pH on enzyme activity.

      Helpful?

  2. What is the optimum temperature of this enzyme?

    1 answer
    1. The optimal temperature is 70 - 75 °C

      Helpful?

  3. Could you please provide the source of the A7095 and the molecular weight of the enzyme? and is this a pure enzyme?

    1 answer
    1. The source of this product can be found on the lot specific Certificate of Origin. This product is from species ASPERGILLUS NIGER. Please access a Certificate of Origin in the DOCUMENTATION section under 'Certificate of Origin':
      https://www.sigmaaldrich.com/product/sigma/a7095#product-documentation

      Amyloglucosidase from A. niger exists as two isoforms with molecular weights of 69,810 kDa and 89,130 kDa, as determined by SDS-PAGE. See: Starch, 34, 346 (1982). The units/mL is determined per batch as reported in Certificate of Analysis. The purity is not part of product specification and is not determined.

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