A7345

Millipore

Anti-V5 Agarose Affinity Gel antibody produced in mouse

purified immunoglobulin, clone V5-10

Synonym(s):
Monoclonal Anti-V5
MDL number:
NACRES:
NA.56

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V5-10, monoclonal

analyte chemical class(es)

proteins (V5)

application(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

capacity

2.5 nmol/mL, gel (V5 fusion protein)

conjugate

agarose conjugate

shipped in

wet ice

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Anti-V5 Agarose Affinity Gel is made of purified immunoglobulin fraction of monoclonal anti-V5 isolated from ascites fluid of the V5-10 hybridoma coupled to cyanogen bromide-activated beaded agarose. The purified monoclonal antibody is immobilized on agarose.
The Anti-V5 Agarose Affinity Gel is composed of the monolonal anti-V5 antibody conjugated to cyanogen bromide-activated beaded agarose. The antibody recognizes the V5-tagged recombinant fusion proteins expressed in transfected mammalian cells.

Specificity

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Application

Anti-V5 Agarose Affinity Gel antibody produced in mouse has been used in immunoprecipitation and recombinant protein purification.

Biochem/physiol Actions

Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide ′affinity handles′ designed to enable the selective identification and purification of the protein of interest. The addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the bioactivity of the protein, or with the biodistribution of the tagged product. Many recombinant proteins have been engineered to express a short sequence derived from the V5 from simian virus known as V5-tag. Anti-“tag” agarose conjugates may facilitate the detection, isolation and purification of these proteins.

Physical form

Suspension of beaded agarose (1:1 v/v) in 0.01 M phosphate buffered saline, containing 15 mM sodium azide.

Storage and Stability

For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Q Meng et al.
Analytical biochemistry, 421(2), 351-361 (2011-11-01)
Quantitation of individual monoclonal antibodies (mAbs) within a combined antibody drug product is required for preclinical and clinical drug development, including pharmacokinetic (PK), toxicology, stability, and biochemical characterization studies of such drugs. We have developed an antitoxin, XOMA 3AB, consisting...
Birger Herzog et al.
Molecular biology of the cell, 22(15), 2766-2776 (2011-06-10)
In endothelial cells, neuropilin-1 (NRP1) binds vascular endothelial growth factor (VEGF)-A and is thought to act as a coreceptor for kinase insert domain-containing receptor (KDR) by associating with KDR and enhancing VEGF signaling. Here we report mutations in the NRP1...
Arko Dasgupta et al.
PLoS genetics, 11(5), e1005215-e1005215 (2015-05-16)
Most organisms on earth sense light through the use of chromophore-bearing photoreceptive proteins with distinct and characteristic photocycle lengths, yet the biological significance of this adduct decay length is neither understood nor has been tested. In the filamentous fungus Neurospora...
Lynnette C Goatley et al.
Journal of virology, 85(7), 3294-3305 (2011-01-21)
The African swine fever virus (ASFV)-encoded CD2v transmembrane protein is required for the hemadsorption of red blood cells around infected cells and is also required for the inhibition of bystander lymphocyte proliferation in response to mitogens. We studied the expression...
Babita Madan et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(21), E2945-E2954 (2016-05-11)
The Wnt signaling pathways play pivotal roles in carcinogenesis. Modulation of the cell-surface abundance of Wnt receptors is emerging as an important mechanism for regulating sensitivity to Wnt ligands. Endocytosis and degradation of the Wnt receptors Frizzled (Fzd) and lipoprotein-related...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service