A7511

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, essentially fatty acid free, ≥97% (agarose gel electrophoresis), 1X Crystallized

Synonym(s):
Albumin bovine serum, Bovine albumin, BSA
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27
Pricing and availability is not currently available.

Quality Level

biological source

bovine

assay

≥97% (agarose gel electrophoresis)

form

lyophilized powder

quality

crystallized

mol wt

~66 kDa

purified by

cold ethanol fractionation

packaging

poly bottle of

origin

USA origin

Fatty acid content

≤0.01%

application(s)

ELISA: suitable
electrophoresis: suitable
western blot: suitable

pH

7

solubility

water: soluble (50 mg/ml)

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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Packaging

1, 5, 10, 25 g in poly bottle

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Essentially fatty acid free
  • Crystallized

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method. Further purified by a proprietary crystallization method
Prepared from albumin (A4378)
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

3

Certificate of Analysis
Certificate of Origin
Julia M Laufer et al.
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Marcos V A S Navarro et al.
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Manoj K Pandey et al.
Molecular pharmacology, 75(3), 525-533 (2008-12-24)
The aim of the current study is to determine whether butein (3,4,2',4'-tetrahydroxychalcone) exhibits antiproliferative effects against tumor cells through suppression of the signal transducer and activator of transcription 3 (STAT3) activation pathway. We investigated the effects of butein on constitutive...
Tânia Veiga et al.
Eukaryotic cell, 11(2), 238-249 (2011-12-14)
The industrial production of penicillin G by Penicillium chrysogenum requires the supplementation of the growth medium with the side chain precursor phenylacetate. The growth of P. chrysogenum with phenylalanine as the sole nitrogen source resulted in the extracellular production of...
Tomo Daidoji et al.
Journal of virology, 82(22), 11294-11307 (2008-09-13)
In recent years, the highly pathogenic avian influenza virus H5N1 has raised serious worldwide concern about an influenza pandemic; however, the biology of H5N1 pathogenesis is largely unknown. To elucidate the mechanism of H5N1 pathogenesis, we prepared primary airway epithelial...

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