A7810

Sigma-Aldrich

Annexin V Biotin Conjugate from human placenta

buffered aqueous solution

Synonym(s):
Calphosbindin I, Lipocortin V, Annexin V, PAP-1
NACRES:
NA.32
Pricing and availability is not currently available.

form

buffered aqueous solution

Quality Level

extent of labeling

3-6 mol biotin per mol annexin V

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... ANXA5(308)

Looking for similar products? Visit Product Comparison Guide

Application

Can be used for detection of apoptotic cells in conjunction with streptavidin coupled to alkaline phosphatase, peroxidase, or FITC.

Biochem/physiol Actions

Annexins are ubiquitous homologous proteins that bind phospholipids in the presence of calcium. The cellular changes involved in the apoptotic process include loss of phospholipid asymmetry during the early stages. This phenomenon is universal and is not limited to stimulus or to mammalian cells, but also occurs in insect and plant cells. In living cells, phosphatidylserine is transported to the inside of the lipid bilayer by the Mg2+ATP dependent enzyme, aminophospholipid translocase. At the onset of apoptosis, phosphatidylserine becomes translocated to the external surface of the cell membrane. Since the movement of phosphatidylserine from the internal membrane surface to the external surface is an early indicator of apoptosis, annexin V and its conjugates that interact strongly and specifically with phosphatidylserine may be used to detect apoptosis. Annexin V conjugates can be used to detect apoptotic cells significantly earlier than DNA-based assays. Fluorescent dye labeled annexin V have applications in flow cytometry, fluorescence microscopy, and laser scanning cytometry.

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, containing 100 mM NaCl.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.