MilliporeSigma
All Photos(5)

Documents

A7888

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, suitable for RIA, pH 5.2, ≥96%

Sign Into View Organizational & Contract Pricing

Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

biological source

bovine

grade

RIA grade

Assay

≥96%

form

lyophilized powder

quality

suitable for RIA

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

blood typing: suitable

loss

≤5%

pH

5.2

solubility

water: soluble (40 mg/ml)

suitability

suitable for insulin RIA procedures

UniProt accession no.

foreign activity

BT virus, none detected
VSV virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A9085A7906A8022
Bovine Serum Albumin heat shock fraction, suitable for RIA, pH 5.2, ≥96%

Sigma-Aldrich

A7888

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, essentially IgG free, ≥96% (agarose gel electrophoresis)

Sigma-Aldrich

A9085

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, pH 7, ≥98%

Sigma-Aldrich

A7906

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, pH 5.2, ≥96%

Sigma-Aldrich

A8022

Bovine Serum Albumin

grade

RIA grade

grade

-

grade

-

grade

-

assay

≥96%

assay

≥96% (agarose gel electrophoresis)

assay

≥98%

assay

≥96%

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

purified by

heat shock fractionation

purified by

heat shock fractionation

purified by

heat shock fractionation

purified by

heat shock fractionation

General description

Bovine serum albumin (BSA) is 66 kDa protein consisting of three domains with two subdomains under each. It is α-helical globular and a non-glycosylated protein.

Application

Bovine Serum Albumin has been used:
  • as a blocking agent in enzyme linked immunosorbent assay (ELISA)(154)
  • as a component of Dulbecco PBS medium for preantral follicles culture(155)
  • as a component of modified Tris-buffered medium (mTBM) for oocyte insemination(156)

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. It is a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates. The cysteine and tyrosine residues of BSA have high affinity for metal binding.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Heat shock fractionated
  • Suitable for radioimmunoassay (RIA)

Caution

May yield an insulin blank of up to 0.1 μunit per mg in certain procedures.

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 8

1 of 8

Lara Vlietstra et al.
PloS one, 14(6), e0217462-e0217462 (2019-06-07)
To determine if there is an association between sarcopenia, physical function and self-reported fatigue in osteoarthritis (OA) and rheumatoid arthritis (RA). A cross-sectional analysis of measurements from a cohort of 157 participants with OA or RA was performed. The relationship
Sampada Chande et al.
Scientific reports, 10(1), 3069-3069 (2020-02-23)
Low blood phosphate (Pi) reduces muscle function in hypophosphatemic disorders. Which Pi transporters are required and whether hormonal changes due to hypophosphatemia contribute to muscle function is unknown. To address these questions we generated a series of conditional knockout mice
Yankel Chekli et al.
Scientific reports, 10(1), 15791-15791 (2020-09-27)
Bacterial proteins exported to the cell surface play key cellular functions. However, despite the interest to study the localisation of surface proteins such as adhesins, transporters or hydrolases, monitoring their dynamics in live imaging remains challenging, due to the limited
K B Cullberg et al.
Nutrition & diabetes, 4, e113-e113 (2014-03-26)
High levels of free fatty acids (FFA) have been suggested to be one of the underlying mechanisms for adipose tissue (AT) inflammation and dysfunction in obesity. Human AT produces several adipokines including monocyte chemoattractant protein-1 (MCP-1), which are involved in
Enzyme-linked immunosorbent assay (ELISA) and blocking with bovine serum albumin (BSA)?not all BSAs are alike
Xiao Y and Isaacs SN
Journal of Immunological Methods, 384(1-2), 148-151 (2012)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service