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A7921

Sigma-Aldrich

Agar

suitable for plant cell culture, powder

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Synonym(s):
Agar-agar, Gum agar
Linear Formula:
(C12H18O9)n
CAS Number:
EC Number:
MDL number:
eCl@ss:
42040102
NACRES:
NA.21

biological source

algae (Rhodophyceae)

Quality Level

form

powder

technique(s)

cell culture | plant: suitable

transition temp

congealing temperature 30-35 °C

application(s)

agriculture

storage temp.

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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This Item
A6686A1296A4550
Agar suitable for plant cell culture, powder

A7921

Agar

Agar Bacteriological, microbiology tested, suitable for plant cell culture, granular

A6686

Agar

Agar microbiology tested, suitable for plant cell culture, suitable for cell culture, powder

A1296

Agar

Agar Type A, suitable for plant cell culture

A4550

Agar

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | mammalian: suitable, cell culture | plant: suitable, microbiological culture: suitable

technique(s)

cell culture | plant: suitable

transition temp

congealing temperature 30-35 °C

transition temp

transition temp 32-39 °C

transition temp

congealing temperature <38 °C (1.5% in H2O)

transition temp

transition temp 32-38 °C (1.5%)

biological source

algae (Rhodophyceae)

biological source

algae (Rhodophyceae)

biological source

algae (Rhodophyceae)

biological source

algae (Rhodophyceae)

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture
agriculture
microbiology
microbiology

application(s)

agriculture

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

General description

Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.

application

Agar has been used as a component of EDMm (modified version of Eucalyptus dunnii basal medium (EDM)) for development of shoots of Eucalyptus dunnii clones. It has also been used as a component of Murashige and Skoog medium for in vitro culture of Strain TC09 of Cladosporium sphaerospermum.
Agar has been used as a component
  • of Gamborg′s B5 regeneration medium
  • of Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds
  • in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings

Biochem/physiol Actions

Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.

Other Notes

Purified for research and protoplast culture.

Preparation Note

Typical working concentration: 6-12 g/L.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)


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25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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Ata Chizari et al.
Biomedical optics express, 11(5), 2352-2365 (2020-06-06)
Functional performance of handheld laser speckle contrast imaging (LSCI) is compromised by movement artefacts. Here we quantify the movements of a handheld LSCI system employing electromagnetic (EM) tracking and measure the applied translational, tilt and on-surface laser beam speeds. By
Bety Shiue de Hsie et al.
Journal of photochemistry and photobiology. B, Biology, 198, 111577-111577 (2019-08-04)
Lippia rotundifolia Cham. is in the family Verbenaceae and is endemic to the Cerrado. This species is aromatic and characterized by the presence of glandular trichomes on its leaves that are rich in monoterpenes. The objective of this study was
Protocol: a method to study the direct reprogramming of lateral root primordia to fertile shoots
Kareem A, et al.
Plant methods, 12(1), 27-27 (2016)
Thidiazuron induced micropropagation of Hypericum triquetrifolium Turra
Oluk, E and Orhan, Serpil
African Journal of Biotechnology, 8(15), 771-777 (2009)
AGAR DOES NOT AFFECT IN VITRO RHIZOGENESIS AND EX VITRO ACCLIMATIZATION OF Handroanthus chrysotrichus
Rabaiolli SMS, et al.
CERNE, 23(2), 185-192 (2017)

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