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A7921

Sigma-Aldrich

Agar

suitable for plant cell culture, powder

Synonym(s):
Agar-agar, Gum agar
Linear Formula:
(C12H18O9)n
CAS Number:
EC Number:
MDL number:
eCl@ss:
42040102
NACRES:
NA.21

Quality Level

biological source

algae (Rhodophyceae)

form

powder

application(s)

cell culture | plant: suitable

transition temp

congealing temperature 30-35 °C

Featured Industry

Agriculture

storage temp.

room temp

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General description

Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.

Application

Agar has been used as a component of EDMm (modified version of Eucalyptus dunnii basal medium (EDM)) for development of shoots of Eucalyptus dunnii clones. It has also been used as a component of Murashige and Skoog medium for in vitro culture of Strain TC09 of Cladosporium sphaerospermum.
Agar has been used as a component
  • of Gamborg′s B5 regeneration medium
  • of Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds
  • in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings

Packaging

2.5 kg in poly drum
1 kg in poly bottle
100, 500 g in poly bottle

Biochem/physiol Actions

Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.

Other Notes

Purified for research and protoplast culture.

Preparation Note

Typical working concentration: 6-12 g/L.

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Zhijian T Li et al.
Frontiers in plant science, 9, 1959-1959 (2019-02-19)
A growing number of bacteria and fungi have been found to promote plant growth through mutualistic interactions involving elements such as volatile organic compounds (VOCs). Here, we report the identification of an environmentally isolated strain of Cladosporium sphaerospermum (herein named...
Plant Tissue Culture, Development, and Biotechnology, 21(11), 1577-1577 (2011)
A new transformation-regeneration procedure in the model legume Lotus japonicus: root explants as a source of large numbers of cells susceptible to Agrobacterium-mediated transformation
Lombari P, et al.
Plant Cell Reports, 21(8), 771-777 (2003)
Thidiazuron induced micropropagation of Hypericum triquetrifolium Turra
Oluk, E and Orhan, Serpil
African Journal of Biotechnology, 8(15), 771-777 (2009)
AGAR DOES NOT AFFECT IN VITRO RHIZOGENESIS AND EX VITRO ACCLIMATIZATION OF Handroanthus chrysotrichus
Rabaiolli SMS, et al.
CERNE, 23(2), 185-192 (2017)

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