A7921

Sigma-Aldrich

Agar

suitable for plant cell culture, powder

Synonym(s):
Agar-agar, Gum agar
Linear Formula:
(C12H18O9)n
CAS Number:
EC Number:
MDL number:
eCl@ss:
42040102
NACRES:
NA.21

Quality Level

biological source

algae (Rhodophyceae)

form

powder

application(s)

cell culture | plant: suitable

transition temp

congealing temperature 30-35 °C

Featured Industry

Agriculture

storage temp.

room temp

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General description

Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.

Application

Agar has been used as a component
  • of Gamborg′s B5 regeneration medium
  • of Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds
  • in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings

Packaging

2.5 kg in poly drum
1 kg in poly bottle
100, 500 g in poly bottle

Biochem/physiol Actions

Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.

Other Notes

Purified for research and protoplast culture.

Preparation Note

Typical working concentration: 6-12 g/L.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Certificate of Analysis

Certificate of Origin

Protocol: a method to study the direct reprogramming of lateral root primordia to fertile shoots
Kareem A, et al.
Plant methods, 12(1), 27-27 (2016)
Bety Shiue de Hsie et al.
Journal of photochemistry and photobiology. B, Biology, 198, 111577-111577 (2019-08-04)
Lippia rotundifolia Cham. is in the family Verbenaceae and is endemic to the Cerrado. This species is aromatic and characterized by the presence of glandular trichomes on its leaves that are rich in monoterpenes. The objective of this study was...
Ata Chizari et al.
Biomedical optics express, 11(5), 2352-2365 (2020-06-06)
Functional performance of handheld laser speckle contrast imaging (LSCI) is compromised by movement artefacts. Here we quantify the movements of a handheld LSCI system employing electromagnetic (EM) tracking and measure the applied translational, tilt and on-surface laser beam speeds. By...
Thidiazuron induced micropropagation of Hypericum triquetrifolium Turra
Oluk, E and Orhan, Serpil
African Journal of Biotechnology, 8(15), 771-777 (2009)
AGAR DOES NOT AFFECT IN VITRO RHIZOGENESIS AND EX VITRO ACCLIMATIZATION OF Handroanthus chrysotrichus
Rabaiolli SMS, et al.
CERNE, 23(2), 185-192 (2017)

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