Repeated freeze/thaw cycles are not recommended for this product; ideally, only one freeze-thaw cycle (for aliquoting) should be performed. Aqueous solutions can be mixed and then frozen for later use, but should be stored in single-use aliquots to avoid repeated freeze/thaw cycles. Stability in solution is not fully determined for this product, but similar products are stable for several months at –20°C.
A8301
Albumin, glycated human
lyophilized powder
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About This Item
Biological source:
human
Assay:
90-100% protein basis (biuret)
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Concentration:
90—100% protein (Biuret)
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SMILES string
FC(F)(F)C(F)(F)C(F)(F)F
InChI
1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11
InChI key
QYSGYZVSCZSLHT-UHFFFAOYSA-N
biological source
human
assay
90-100% protein basis (biuret)
form
lyophilized powder
concentration
90—100% protein (Biuret)
extent of labeling
1-5 mol hexose (as fructosamine) per mol albumin
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... ALB(213)
1 of 4
This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) ELISA: suitable, tissue culture: suitable, western blot: suitable | technique(s) protein quantification: suitable | technique(s) RNA extraction: suitable |
| biological source human | biological source human | biological source - | biological source human |
| assay 90-100% protein basis (biuret) | assay ≥96% (agarose gel electrophoresis) | assay ≥95% (SDS-PAGE) | assay - |
| Quality Level 200 | Quality Level 300 | Quality Level - | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized solid (Salt-free) | form lyophilized powder |
| Gene Information human ... ALB(213) | Gene Information human ... ALB(213) | Gene Information - | Gene Information human ... ALB(213) |
General description
Albumin gene is mapped to human chromosome 4q13.3. It is synthesized as a preproalbumin with 609 amino acids in the liver. The mature albumin comprising 585 residues, is generated by the cleavage of signal peptide. Albumin is the major circulating protein in serum. It comprises three domains (I, II and III), each of which is subdivided into two subdomains A and B. A total of six ligand binding site is associated with albumin. Domain IIA and IIIA harbor small heterocyclic or aromatic compounds binding site. Long-chain fatty acid binding sites is present in IB and IIIB domains.
Application
Naturally occurring glycated albumin is a sensitive indicator of glycemic control by diabetic patients.
Albumin, glycated human has been used:
- as a component of serum-free medium for retinal microglia activation
- to stimulate immortalised proximal tubule epithelial (HK-2) cells for fibronectin production
- as medium supplement in human retinal microvascular endothelial cells (HRMECs) cultures to test its effect on family with sequence similarity 18, member B (FAM18B) expression
Biochem/physiol Actions
Albumin is crucial for physiological processes and is the major transporter for lipids, metal ions and amino acids. It has antioxidant functionality and binds to drugs as well. Mutation in the albumin gene is implicated in an autosomal recessive disorder called Analbuminemia, which is characterized by low levels of albumin. The major glycation site is lysine 525 in albumin. Glycated albumin is a marker of glycemia in diabetes mellitus patients and is useful in assessment of HbA1c based glycemic control.
Physical form
Lyophilized powder containing citrate buffer salts
Preparation Note
Glycated in vitro
Other Notes
View more information on human serum albumin.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Role of FAM18B in diabetic retinopathy
Wang AL, et al.
Molecular Vision, 20(1), 1146-1146 (2014)
Mizuho Nakayama et al.
Nature communications, 11(1), 2333-2333 (2020-05-13)
Missense-type mutant p53 plays a tumor-promoting role through gain-of-function (GOF) mechanism. In addition, the loss of wild-type TP53 through loss of heterozygosity (LOH) is widely found in cancer cells. However, malignant progression induced by cooperation of TP53 GOF mutation and
Kevin P O'Rourke et al.
Nature biotechnology, 35(6), 577-582 (2017-05-02)
Colorectal cancer (CRC) is a leading cause of death in the developed world, yet facile preclinical models that mimic the natural stages of CRC progression are lacking. Through the orthotopic engraftment of colon organoids we describe a broadly usable immunocompetent
Stephanie D Burr et al.
Cells, 10(3) (2021-04-04)
Cardiovascular disease, specifically heart failure, is a common complication for individuals with type 2 diabetes mellitus. Heart failure can arise with stiffening of the left ventricle, which can be caused by "active" cardiac fibroblasts (i.e., myofibroblasts) remodeling the extracellular matrix
A novel mutation in the albumin gene (c. 1A> C) resulting in analbuminemia
Caridi G, et al.
European Journal of Clinical Investigation, 43(1), 72-78 (2013)
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How many freeze/thaw cycles are permissible? Can aqueous solutions be mixed and then frozen for later use?
1 answer-
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How should this product be stored once reconstituted?
1 answer-
The stability of this product in solution has not been determined. Non-glycated Human Serum Albumin (HSA) solution products are stable for at least 1 year when filter-sterilized and stored refrigerated.
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What is the carbohydrate used for the glycation step of Product A8301, Albumin, glycated human?
1 answer-
The starting carbohydrate for the albumin modification is glucose.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How much is Product A8301, Albumin, glycated human, glycated?
1 answer-
The degree of glycation varies with each individual lot. This value can be found on the Certificate of Analysis for each lot.
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What is the molecular weight of Product A8301, Albumin, glycated human?
1 answer-
Because the degree of glycation varies with each lot, the calculated molecular weight (MW) varies with each lot. The parent albumin has a molecular weight of approximately 66,478 Daltons. Each hexose unit adds approximately 180 Daltons to the molecular weight.
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