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A8311

Sigma-Aldrich

Antifoam 204

aqueous emulsion for bacterial and mammalian systems

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Synonym(s):
organic antifoam
MDL number:
NACRES:
NA.85

Quality Level

form

emulsion (aqueous)

concentration

100% (organic)

density

1.01 g/mL at 25 °C

application(s)

microbiology

storage temp.

room temp

suitability

(Mammalian (suspension))
bacteria (fermentation)

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This Item
A6426A6582A8011
Sigma-Aldrich

Sigma-Aldrich

A8311

Antifoam 204

Sigma-Aldrich

Sigma-Aldrich

A6426

Antifoam 204

Antifoam A concentrate active silicone polymer 100%

Sigma-Aldrich

A6582

Antifoam A concentrate

Antifoam C Emulsion aqueous-silicone emulsion

Sigma-Aldrich

A8011

Antifoam C Emulsion

application(s)

microbiology

application(s)

-

application(s)

microbiology

application(s)

-

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

-

suitability

(Mammalian (suspension)), bacteria (fermentation)

suitability

-

suitability

(Mammalian (suspension)), bacteria (fermentation)

suitability

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

concentration

100% (organic)

concentration

-

concentration

-

concentration

-

General description

Antifoam 204 is an organic antifoam used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Application

Suitable for preventing or minimizing foaming in microbial fermentation systems and mammalian suspension cultures.
Antifoam 204 has been used:
  • in the expression and purification of PUL (PLAP, Ufd3p, and Lub1p) domain
  • in fed-batch fermentation
  • to prevent bubble formation due in aeration in Luria Bertani (LB) culture medium for bacterial growth
  • in the batch fermentation with mineral medium

Components

Antifoam 204 is a 100% active components and a mixture of non-silicone organic defoamers in a polyol dispersion.

Other Notes

Contains 100% active components and is a mixture of non-silicone organic defoamers in a polyol dispersion. Can be sterilized repeatedly.

Preparation Note

For use in microbiological media Sigma recommends a starting concentration of between 0.005% and 0.01%.
The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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