A8354

Sigma-Aldrich

Monoclonal Anti-β-Amyloid antibody produced in mouse

clone NAB 228, purified from hybridoma cell culture

Synonym(s):
Anti-Aβ
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

NAB 228, monoclonal

form

buffered aqueous solution

mol wt

antigen ~110 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

application(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using cell extract of the human embryonal carcinoma NTERA-2 (NT2/D1) cells, treated for 2-3 weeks with 10 μM retinoic acid

isotype

IgG2a

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... APP(351)

General description

The β-amyloid precursor protein (APP) is cleaved sequentially by the proteolytic enzymes β-secretase (BACE1) and γ-secretase to produce β-amyloid (Aβ) peptides with the Aβ1-42 and the Aβ1-40 forms being the most prevalent. Secreted Aβ peptides are degraded either via a re-uptake mechanism followed by endosomal degradation, or by an extracellular insulin degrading enzyme. Extracellular accumulation of Aβ leads to the formation of aggregates, fibrils and eventually amyloid deposits called neuritic plaques, which is the hallmark of Alzheimer′s disease (AD).
The antibody recognizes human β-amyloid peptide, full-length amyloid precursor protein (APP), soluble-APP (sAPPβ′ and sAPPα), C99 cleavage form, and Aβ (1-40/42), but not soluble-APP form sAPPβ.
Amyloid β-peptide (Aβ), a 38 to 43 amino acid peptide is obtained from the β-amyloid precursor protein (APP). β-amyloid gene is located on human chromosome 21q21. APP is a type I transmembrane protein. It is produced in the endoplasmic reticulum (ER) and then migrated with the help of Golgi apparatus to the trans-Golgi-network (TGN).

Immunogen

synthetic peptide corresponding to amino acids 1-11 of human β-amyloid protein.

Application

Mouse Monoclonal Anti-β-Amyloid antibody has been used for western blot assays. The product can also be used for immunohistochemistry, immunoprecipitation, indirect ELISA and microarray studies.
Monoclonal Anti-β-Amyloid antibody has been used in the titration of IgG1 and IgG2a isotype antibodies. It has also been used in the synthesis of antibody-functionalized magnetic nanoparticles.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

β-amyloid gene acts as the substrate of insulin-degrading enzyme (IDE). It plays a major role in the pathogenesis of Alzheimer′s disease (AD) and type 2 diabetes mellitus (DM2).

Target description

Amyloids are insoluble protein aggregates consisting of misfolded proteins and peptides. Amyloid deposition are associated with multiple neurodegenerative disorders.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Do you have a picture of the western blot including the amount of protein loaded and antibody dilution used for Product A8354 - Monoclonal Anti-β-Amyloid antibody produced in mouse?

    Attaced is a QC work sheet that includes al the information your customer required. the extract used is NT2 (human embryonal Carcinoma) cells treated with 10 micromolar Retinoic acid for 2-3 weeks

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

APP processing in Alzheimer's disease
Zhang Y W, et al.
Molecular Brain, 4(1), 3-3 (2011)
Edward B Lee et al.
The Journal of biological chemistry, 278(7), 4458-4466 (2002-12-14)
Insoluble pools of the amyloid-beta peptide (Abeta) in brains of Alzheimer's disease patients exhibit considerable N- and C-terminal heterogeneity. Mounting evidence suggests that both C-terminal extensions and N-terminal truncations help precipitate amyloid plaque formation. Although mechanisms underlying the increased generation...
Detection of plasma biomarkers using immunomagnetic reduction: a promising method for the early diagnosis of Alzheimer?s disease
Yang S Y, et al.
Neurology and therapy, 6(1), 37-56 (2017)
Insulin-degrading enzyme regulates the levels of insulin, amyloid β-protein, and the β-amyloid precursor protein intracellular domain in vivo.
Farris W, et al.
Proceedings of the National Academy of Sciences of the USA, 100(7), 4162-4167 (2003)
Xi-Jun Song et al.
Molecular biology reports, 47(3), 2047-2059 (2020-02-10)
Alzheimer's disease (AD) is a neurodegenerative disorder, and the pathological mechanism of the disease is still far to understand. According to the amyloid cascade hypothesis in AD, Amyloid-β (Aβ) is considered as a key substance that contributes AD development. Aβ...

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