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A8412

Sigma-Aldrich

Bovine Serum Albumin solution

7.5% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

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Synonym(s):
Albumin solution from bovine serum, BSA solution
CAS Number:
MDL number:
NACRES:
NA.75

biological source

bovine

description

low endotoxin

sterility

sterile-filtered

product line

BioXtra

Assay

7.0-8.0% protein basis (UV)

form

liquid

mol wt

66.4 kDa

purified by

cold ethanol and heatshock fractionation

origin

USA origin

concentration

7.5% in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

≤13.0 EU/mL Endotoxin level

pH

6.5-7.5

foreign activity

Mycoplasma test (Negative)
cell growth >50%

shipped in

ambient

storage temp.

2-8°C

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This Item
A9576A1662A1595
sterility

sterile-filtered

sterility

sterile-filtered

sterility

aseptically filled

sterility

sterile-filtered

assay

7.0-8.0% protein basis (UV)

assay

28-32% total protein basis (UV)

assay

28-32% protein basis (biuret)

assay

90-120 mg/mL protein basis (biuret)

form

liquid

form

liquid

form

solution

form

solution

mol wt

66.4 kDa

mol wt

-

mol wt

-

mol wt

-

purified by

cold ethanol and heatshock fractionation

purified by

-

purified by

heat shock fractionation

purified by

heat shock fractionation

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

Qualified for use in cell culture applications.

Biochem/physiol Actions

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media (SFM). It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Pinar Sahin et al.
Fertility and sterility, 102(5), 1482-1490 (2014-09-23)
To evaluate the expression of mammalian target of rapamycin (mTOR) pathway molecules in mouse spermatogenesis and as well as its role during proliferation and meiotic initiation of spermatogenic cells. Experimental animal study. University. C57Balb-C adult male mice. Expressions of mTOR
Amanda J Shapiro et al.
Veterinary microbiology, 177(1-2), 154-161 (2015-03-18)
The seroprevalence of Coxiella burnetii (C. burnetii) in cats in eastern Australia is unknown, and the risk of transmission from cats to humans is undetermined. This study aimed to determine the exposure of cats to C. burnetii in four distinct
Paul J Orchard et al.
Scientific reports, 9(1), 7858-7858 (2019-05-28)
Cerebral adrenoleukodystrophy (cALD) is an inflammatory neurodegenerative disease associated with mutation of the ABCD1 gene. Proteomic analysis of cerebral spinal fluid (CSF) from young males with active cALD revealed markers of inflammation including APOE4. APOE4 genotype has been associated with
Eric M Kernfeld et al.
Immunity, 48(6), 1258-1270 (2018-06-10)
Thymus development is critical to the adaptive immune system, yet a comprehensive transcriptional framework capturing thymus organogenesis at single-cell resolution is still needed. We applied single-cell RNA sequencing (RNA-seq) to capture 8 days of thymus development, perturbations of T cell receptor rearrangement
Kristen Johnson et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 32(10), 2062-2073 (2017-06-11)
Fibroblast growth factor 23 (FGF23) is the causative factor of X-linked hypophosphatemia (XLH), a genetic disorder effecting 1:20,000 that is characterized by excessive phosphate excretion, elevated FGF23 levels and a rickets/osteomalacia phenotype. FGF23 inhibits phosphate reabsorption and suppresses 1α,25-dihydroxyvitamin D

Articles

Albumin in Cell Culture

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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