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MilliporeSigma

A8412

Sigma-Aldrich

Bovine Serum Albumin solution

7.5% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

Synonym(s):

Albumin solution from bovine serum, BSA solution

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100 ML
$67.30

About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.75

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$67.30


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biological source

bovine

Quality Level

description

low endotoxin

sterility

sterile-filtered

product line

BioXtra

assay

7.0-8.0% protein basis (UV)

form

liquid

mol wt

66.4 kDa

purified by

cold ethanol and heatshock fractionation

origin

USA origin

concentration

7.5% in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

≤13.0 EU/mL Endotoxin level

pH

6.5-7.5

foreign activity

Mycoplasma test (Negative)
cell growth >50%

shipped in

ambient

storage temp.

2-8°C

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Show Differences

1 of 4

This Item
A7979A9576A3174
biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

technique(s)

cell culture | mammalian: suitable

technique(s)

blocking: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

blocking: suitable

assay

7.0-8.0% protein basis (UV)

assay

33-37% total protein basis (UV)

assay

28-32% total protein basis (UV)

assay

28-32% (biuret)

Quality Level

200

Quality Level

400

Quality Level

300

Quality Level

200

form

liquid

form

liquid

form

liquid

form

solution

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

Qualified for use in cell culture applications.

Biochem/physiol Actions

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media (SFM). It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Questions

1–3 of 3 Questions  
  1. Is this product RNAse/DNAse free? CAS No. 9048-46-8

    1 answer
    1. Unfortunately, this product is not tested for RNAse/DNAse activity. Please see the link below to review product B8667, which is an RNase/DNase-free BSA solution:
      https://www.sigmaaldrich.com/product/sigma/b8667

      Helpful?

  2. Is this product fatty acid free?

    1 answer
    1. This product is not fatty acid free, it is fatty-acid depleted. The product is not tested for free fatty acid content. Please see several options below for fatty-acid free BSAs offered:
      https://www.sigmaaldrich.com/product/sigma/a8806
      https://www.sigmaaldrich.com/product/sigma/a7030
      https://www.sigmaaldrich.com/product/sigma/a1595
      https://www.sigmaaldrich.com/product/mm/126609

      Helpful?

  3. La référence A8412 a t-elle une date de retest ou d'expiration donnée par le fournisseur svp ?

    1 answer
    1. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

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