A8542

Sigma-Aldrich

Anti-Human IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

MDL number:
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

goat

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

application(s)

direct ELISA: 1:40,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot (chemiluminescent): 1:30,000

conjugate

alkaline phosphatase conjugate

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

The conjugate is specific for human IgG and human IgG Fab fragment. The conjugate shows no reactivity with human IgG Fc fragment, mouse IgG or rat IgG.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG.
Antiserum is developed in goat using purified human IgG Fab fragment as the immunogen. Antibody is isolated from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Goat anti-human IgG is conjugated to Alkaline Phosphatase by protein cross linking with 0.2% glutaraldehyde
Specificity of the Alkaline Phosphatase Conjugated Anti-Human IgG is determined by Enzyme Linked Immunosorbent Assay (ELISA). Cross reactivity of the antibody-conjugate is also determined by ELISA
Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation.

Specificity

Binds all human Igs.
Provides reduced background staining with mouse or rat samples.

Immunogen

Developed in goat using purified human IgG Fab fragment

Application

Anti-Human IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA) and chemiluminescent ELISA.
Nuclear proteins isolated from primary neuronal cells were analyzed by western blot using alkaline phosphatase-conjugated goat anti-human Fab specific as the secondary antibody.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Other Notes

No cross-reaction with mouse and rat IgG.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Hazard Codes

Xn

Risk Statement

21/22

Safety Statement

36/37

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Michelle R Ugolini-Lopes et al.
Advances in rheumatology (London, England), 59(1), 19-19 (2019-05-16)
To evaluate the frequency of anti-collagen type V in humans with early systemic sclerosis (SSc) compared to defined SSc patients and healthy controls, since collagen type V was shown to be overexpressed in early SSc patients' skin and there is...
Gerald S Baron et al.
The Journal of biological chemistry, 278(17), 14883-14892 (2003-02-21)
Prion protein (PrP) is usually bound to membranes by a glycosylphosphatidylinositol (GPI) anchor that associates with detergent-resistant membranes, or rafts. To examine the effect of membrane association on the interaction between the normal protease-sensitive PrP isoform (PrP-sen) and the protease-resistant...
Evaluation of a latex agglutination kit for the detection of human antibodies to the lipopolysaccharide of Escherichia coli O157, following infection with verocytotoxin-producing E. coli O157
Chart H
Letters in Applied Microbiology, 29(6), 434-436 (1999)
Pharmacokinetics and immunologic consequences of exposing macaques to purified homologous butyrylcholinesterase
Rosenberg Y, et al.
Life Sciences, 72(2), 125-134 (2002)
Molecular Genetics of Immunoglobulin (1987)

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