The conjugate is specific for human IgG and human IgG Fab fragment. The conjugate shows no reactivity with human IgG Fc fragment, mouse IgG or rat IgG.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG.
Antiserum is developed in goat using purified human IgG Fab fragment as the immunogen. Antibody is isolated from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Goat anti-human IgG is conjugated to Alkaline Phosphatase by protein cross linking with 0.2% glutaraldehyde
Specificity of the Alkaline Phosphatase Conjugated Anti-Human IgG is determined by Enzyme Linked Immunosorbent Assay (ELISA). Cross reactivity of the antibody-conjugate is also determined by ELISA
Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation.
Binds all human Igs.
Provides reduced background staining with mouse or rat samples.
Developed in goat using purified human IgG Fab fragment
Anti-Human IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA) and chemiluminescent ELISA.
Nuclear proteins isolated from primary neuronal cells were analyzed by western blot using alkaline phosphatase-conjugated goat anti-human Fab specific as the secondary antibody.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.
No cross-reaction with mouse and rat IgG.
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
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