A8592

Sigma-Aldrich

Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):
Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG M2 antibody produced in mouse
NACRES:
NA.32

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

concentration

~1 mg/mL

application(s)

indirect ELISA: 1:20,000

isotype

IgG1

immunogen sequence

DYKDDDDK

conjugate

peroxidase conjugate

shipped in

wet ice

storage temp.

−20°C

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General description

The Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides.

Packaging

0.2, 1 mg in poly bottle

Application

Suggested dilution for immunocytochemistry and western blotting 1:100 to 1:1000
Suggested dilution for ELISA 1:20,000

Browse additional application references in our
FLAG® Literature portal.
For simple, one-step detection by immunocytochemistry, ELISA, or Western blotting. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity.

Physical form

Solution in phosphate buffered saline containing 50% glycerol plus preservative and stabilizer

Preparation Note

Dilute ANTI-FLAG M2-Peroxidase solution in Tris Buffered Saline (TBS): 0.05 M Tris, pH 7.4, with 0.15 M NaCl.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
  1. Can product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, be used for immunohistochemistry?

    The product cannot be used on paraffin embedded tissue sections, but it does work on fixed culture cells (as indicated on the Product Information Sheet) and also may work on frozen sections.

  2. What concentration of Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, is recommended for my application?

    An antibody titer from 1:100 to 1:1000 is recommended for immunocytochemistry and Western blot.  Minimum antibody titer recommended for ELISA is 1:20,000.  Adjust antibody titer to maximize detection sensitivity and to minimize background.

  3. What are the appropriate storage conditions for Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody?

    This product ships on wet ice and it is recommended to store the undiluted antibody at 0 to -20°C.  Once diluted, repeated freezing and thawing is not recommended.

  4. I am getting a high background on my Western blots using Product No. A8592, Monoclonal Anti-FLAG® M2-Peroxidase (HRP) antibody.  What can I do to fix this?

    There is a troubleshooting guide provided at the end of the product information sheet.  Some suggestions include the following:  adjusting antibody titer, increase number of washes, increase stringency of washes by addition of 0.05% TWEEN 20 or 0.1% TRITON X-100 to the wash buffers, and/or increase the incubation and wash temperatures. 

  5. How is Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, supplied?

    Product No. A8592, ANTI-FLAG M2-peroxidase is supplied as a solution in 10 mM sodium phosphate, pH 7.4, containing 150 mM NaCl and 50% glycerol, plus stabilizer and preservative.  The conjugate protein concentration is approximately 1 mg/ml.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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