A8667

Sigma-Aldrich

Anti-Human IgG (whole molecule)−Peroxidase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

MDL number:
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

goat

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

application(s)

direct ELISA: 1:30,000
dot blot: 1:80,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

conjugate

peroxidase conjugate

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Goat Anti-Human IgG (whole molecule)-Peroxidase antibody binds to human IgG.

Immunogen

human IgG

Application

Specificities of anti-human HIV-1 envelope antibodies was determined by ELISA using HRP-conjugated goat anti-human IgG at a dilution of 1:1000.
Goat Anti-Human IgG (whole molecule)-Peroxidase antibody has been used for ELISA, dot blot and immunohistochemistry.
Anti-Human IgG (whole molecule)-Peroxidase antibody produced in goat has also been used as a conjugate for gold nanoparticles (AuNPs) in electrochemical detection.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Hui Wang et al.
Virology journal, 8, 539-539 (2011-12-16)
Kaposi sarcoma (KS) is a complex multifocal neoplasm and is the major cause of death for about 50% of acquired immunodeficiency syndrome (AIDS) patients. Kaposi's sarcoma-associated herpesvirus (KSHV) is an oncogenic virus with a causal role in the development of...
L D Curtis et al.
Journal of thrombosis and haemostasis : JTH, 3(6), 1180-1186 (2005-05-17)
BB-10153 is an engineered variant of human plasminogen that is activated to plasmin by thrombin. Thrombus-selective induction of reperfusion and prevention of reocclusion have been demonstrated following bolus administration in animal models of thrombosis. The objective of the study was...
Cairen Chen et al.
FEBS open bio, 6(3), 211-215 (2016-04-06)
A recent study reported that circulating antibodies to CD25-derived peptide antigens were significantly higher in patients with nonsmall cell lung cancer (NSCLC) than control subjects. The present study was, thus, undertaken to replicate the initial finding with different sample sets....
Yingji Jin et al.
Oncotarget, 8(62), 105425-105439 (2017-12-30)
Serum autoantibodies against tumor-associated antigens (TAAs) have received much attention as potential biomarkers for early detection of cancers, since they can be detected in the early stages of cancers. Autoantibodies against Cancer Antigen 15-3 (CA15-3), carcinoembryonic antigen (CEA), Cancer Antigen...
S Metcalfe et al.
Breast cancer research : BCR, 2(6), 438-443 (2000-11-01)
Serial plasma samples from 1006 patients with breast cancer revealed: (i) no correlation of p53 autoantibody status with disease status at the time of sample collection, or with menopausal status at time of primary diagnosis of breast cancer; (ii) 155...
Articles
Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.
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