Documents

A8850

Sigma-Aldrich

Auto-Activated Protein Kinase from bovine kidney

from bovine kidney, aqueous solution

Synonym(s):
AK
MDL number:
NACRES:
NA.32

Quality Level

biological source

bovine kidney

description

Minimum 50 ng protein/vial by Bradford

form

aqueous solution

specific activity

~17,000 units/mg protein

mol wt

36 kDa

shipped in

dry ice

storage temp.

−70°C

Biochem/physiol Actions

This enzyme phosphorylates and inactivates protein phosphatase 2A. The funtion appears to be related to the catalytic domain of p21-activated p65(PAK) protein kinase which is produced in apoptotic cells. AK is also involved in cytoskeleton organization and other signal transduction processes.

Unit Definition

One unit is the amount of enzyme that will incorporate 1 nmole of phosphoryl groups using myelin basic protein as a substrate per minute at pH 7 at 30 deg C.

Physical form

50 ng per vial in 10 μl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-Mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, 65% ethyleneglycol, and 0.1% Brij® 35.

Legal Information

Brij is a registered trademark of Croda International PLC

WGK Germany

1

Certificate of Analysis
Certificate of Origin

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