A9044

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti Mouse Antibody, Anti Mouse Hrp Sigma, Anti Mouse Hrp, Sigma Anti Mouse Hrp, Anti Mouse Antibody - Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit, Anti Mouse Igg
MDL number:
NACRES:
NA.46

biological source

rabbit

Quality Level

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

application(s)

direct ELISA: 1:40,000 using using 5 μg/ml of mouse IgG for coating and OPD substrate
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot (chemiluminescent): 1:80,000-160,000

conjugate

peroxidase conjugate

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.
IgG antibody is a glycoprotein, composed of two polypeptide chains (light and heavy), each possessing variable and constant domains. The variable region of IgG antibody is specific to antigens and is highly conserved.
Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for all mouse IgG subclasses. IgG is purified from mouse serum by fractionation and ion exchange chromatography. The purified IgG is then conjugated to peroxidase by protein cross-linking with 0.2% glutaraldehyde.

Specificity

Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for all mouse IgG subclasses.

Immunogen

Purified mouse IgG

Application

Epidermis protein extracts were generated from human mammary surgery specimens and subjected to western blot using HRP-conjugated rabbit anti-mouse IgG as the secondary at a 1:80000 dilution in TBSt/5% milk.
Anti-Mouse IgG (whole molecule)–Peroxidase antibody may be used in direct ELISA at a working antibody dilution of 1:40,000. For immunoblotting, a dilution of 1:80,000 to 1:160,000 may be used. A working antibody dilution of 1:200 is recommended for immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil or human appendix sections. Mouse IgG-peroxidase antibody was used at a dilution of 1:20 for immunohistochemistry of mice muscle tissue sections; a dilution of 1:500 was used for South-western blotting in the same study. For immunoblotting of human skeletal muscle tissue extracts, a dilution of 1:5000 was used.
Anti-Mouse IgG (whole molecule)–Peroxidase antibody has been used in western blotting , immunohistochemistry and protein pin array assay.

Biochem/physiol Actions

IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody has its function similar to IgM antibody in complement system activation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Storage and Stability

Store at -20 °C for long term. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots -20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

mRNA and protein expression for novel GABA A receptors θ and ρ2 are altered in schizophrenia and mood disorders; relevance to FMRP-mGluR5 signaling pathway.
Fatemi SH, et al.
Translational Psychiatry, 3(6), e271-e271 (2013)
The Immunoglobulins: Structure and Function (1998)
Anti-inflammatory activity of immunoglobulin G resulting from Fc sialylation
Kaneko Y, et al.
Science, 313(5787), 670-673 (2006)
The Laboratory Rat (2000)
Rita Ferreira et al.
European journal of applied physiology, 97(3), 340-346 (2006-06-14)
The comprehension of the cellular mechanisms underlying skeletal muscle atrophy has been the aim of several experimental studies. However, the majority of them focused on alterations of the myocytes induced by different experimental conditions yet disregarding the contribution of other...

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