A9045

Sigma-Aldrich

Agarose, low gelling temperature

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

Synonym(s):
2-Hydroxyethyl agarose
CAS Number:
MDL number:
NACRES:
NA.21

Quality Level

product line

BioReagent

form

powder

application(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

EEO

≤0.1

mp

≤65 °C ( at a 1.5% gel)

transition temp

congealing temperature 26-30 °C

gel strength

≥200 g/cm2 (1% gel)

solubility

H2O: 10 mg/mL (with heat)

anion traces

sulfate (SO42-): ≤0.10%

foreign activity

RNase and DNase free

Related Categories

General description

Agarose is an algal polysaccharide. Agarose is a thermoreversible, ion-dependent gelling agent. Agarose gel electrophoresis is useful for the clinical routine analyses of proteins in plasma and other body fluids. It is a low gelling temperature derivative with unique gelling properties. Gels forms at <30°C, remelt at temperatures in excess of 60°C. Gels exhibit excellent clarity and are particularly useful for the preparation of media containing heat-labile materials.

Application

Agarose, a low gelling temperature derivative, is the suitable reagent for:
  • plant cell culture studies
  • insect cell culture studies
  • cell culture studies

It may be used for the following studies:
  • Recovery of defined RNA and DNA fractions after electrophoretic separation.
  • Cytochemical staining procedure to investigate the succinate dehydrogenase (SDH) activity in pre-ovulatory mouse oocytes.
  • Purification of RNA in Caenorhabditis elegans by electrophoresis.

Packaging

5, 10, 25, 50, 100, 250 g in poly bottle

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I use Agarose, low gelling temperature?

    This product forms a suspension in nearly any aqueous buffer. Heating will melt the agar, which, upon cooling, will form a gel.

  4. Can Agarose, low gelling temperature, be autoclaved?

    This agarose can be autoclaved.

  5. What is the gel strength of Agarose, low gelling temperature?

    We have a specification of not less than 200 g/cm2 for a 1.0% agarose gel.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

M L Boerjan et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 39(1), 135-138 (1991-01-01)
We describe a cytochemical staining procedure for succinate dehydrogenase (SDH) activity in pre-ovulatory mouse oocytes. The oocytes were embedded in low gelling temperature agarose and treated with caffeine before cytochemical staining in the presence of nitro blue tetrazolium (NBT), phenazinemethosulfate...
Patrick M Ferree et al.
Scientific reports, 9(1), 12194-12194 (2019-08-23)
Males of hymenopteran insects, which include ants, bees and wasps, develop as haploids from unfertilized eggs. In order to accommodate their lack of homologous chromosome pairs, some hymenopterans such as the honeybee have been shown to produce haploid sperm through...
Nariko Arimura et al.
Science advances, 6(36) (2020-09-13)
For normal neurogenesis and circuit formation, delamination of differentiating neurons from the proliferative zone must be precisely controlled; however, the regulatory mechanisms underlying cell attachment are poorly understood. Here, we show that Down syndrome cell adhesion molecule (DSCAM) controls neuronal...
A Fire et al.
Nature, 391(6669), 806-811 (1998-03-05)
Experimental introduction of RNA into cells can be used in certain biological systems to interfere with the function of an endogenous gene. Such effects have been proposed to result from a simple antisense mechanism that depends on hybridization between the...
Agarose gel electrophoresis.
Johansson B G.
Scandinavian Journal of Clinical and Laboratory Investigation, 29(S124), 7-19 (1972)

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