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A9099

Sigma-Aldrich

Acrylamide

for molecular biology, ≥99% (HPLC)

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Synonym(s):
2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide
Linear Formula:
CH2=CHCONH2
CAS Number:
Molecular Weight:
71.08
Beilstein/REAXYS Number:
605349
EC Number:
MDL number:
eCl@ss:
39031205
PubChem Substance ID:
NACRES:
NA.31

grade

for molecular biology

Quality Level

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

assay

≥99% (HPLC)

form

crystals

impurities

≤0.005% Acrylic acid content
≤0.005% Water insolubles

conductivity

≤10 μmho (35% solution)

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

cation traces

Fe: ≤1 ppm
Mg: ≤3 ppm
heavy metals (as Pb): ≤5 ppm

foreign activity

DNase, RNase, protease, none detected

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

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1 of 4

This Item
01696A888708267
vibrant-m

A9099

Acrylamide

-
vibrant-m

01696

Acrylamide

Essential+ Grade
vibrant-m

A8887

Acrylamide

-
vibrant-m

08267

Acrylamide

-
form

crystals

form

powder blend

form

crystals

form

-

foreign activity

DNase, RNase, protease, none detected

foreign activity

-

foreign activity

-

foreign activity

-

impurities

≤0.005% Acrylic acid content, ≤0.005% Water insolubles

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

-

impurities

-

vapor density

2.45 (vs air)

vapor density

2.45 (vs air)

vapor density

2.45 (vs air)

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide. Acrylamide is a water-soluble monomer, which forms a transparent stable insoluble gel. Bis-acrylamide is a cross-linking agent.

Application

Acrylamide has been used:
  • in polyacrylamide gel electrophoresis
  • in the preparation of cleared gel samples
  • to determine the effect of L-cysteine on acrylamide-induced testicular toxicity

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

280.4 °F - closed cup

flash_point_c

138 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Ana Techniqs in Biotechnology (2011)
Separation of Thylakoid Protein Complexes with Two-dimensional Native-PAGE.
Rantala M, et al.
The Plant Journal, 8(13), 29089-29089 (2017)
Electrical characterization of DNA supported on nitrocellulose membranes.
Al AM, et al.
Scientific Reports, 6(4), 29089-29089 (2016)
Mild myelin disruption elicits early alteration in behavior and proliferation in the subventricular zone.
Gould EA,et al.
eLife, 7(4-5), e34783-e34783 (2018)
L-Cysteine Partially Protects Against Acrylamide-Induced Testicular Toxicity.
Kacar S, et al.
Balkan medical journal, 35(4), 311-311 (2018)

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