A9205

Sigma-Aldrich

Bovine Serum Albumin solution

30% in saline, fatty acid free, aseptically filled

Synonym(s):
BSA solution, Albumin solution from bovine serum
CAS Number:
MDL number:
NACRES:
NA.27

Quality Level

biological source

bovine

sterility

aseptically filled

assay

28-32% protein basis (biuret)

form

solution

purified by

cold ethanol fractionation

does not contain

stabilizer or preservative

origin

USA origin

concentration

30% in saline

free fatty acid content

≤0.01%

application(s)

ELISA: suitable
electrophoresis: suitable
western blot: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Bovine Serum Albumin (BSA) has three homologous domains, each subdivided it two domain (A and B). It is a α-helical globular protein and shares structural features with human serum albumin.

Application

Bovine Serum Albumin solution has been used:
  • as Dulbecco′s modified Eagle′s medium supplement for culturing HepG2 human liver cancer cells
  • as a component of hepatocyte recovery medium
  • as a component of fusion and activation buffers for in vitro reconstructed sheep embryos

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the major protein present in blood and has calcium binding sites and regulates metal homeostasis. It is a crucial component of the cell culture medium and promotes embryonic stem cells (hESC) differentiation. Due to its high solubility, low cost and purity it is widely used in cosmetic and pharmaceutical industry. BSA is used as a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates.

Preparation Note

Initial fractionation by cold alcohol precipitation

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Effects of different states of sheep fetal fibroblasts as donor cells on the early development in vitro of reconstructed sheep embryos
Wang H, et al.
Science in China. Series C, Life Sciences / Chinese Academy of Sciences, 50(2), 178-185 (2007)
Jing-Syuna Ruan et al.
The Journal of nutritional biochemistry, 59, 37-48 (2018-07-01)
Lipotoxicity is associated with a high level of fatty acid accumulation in pancreatic β-cells. An overload of free fatty acids contributes to pancreatic β-cell apoptosis and dysfunction. Insulin secretion involves sequential ionic events upon glucose stimulation. ATP sensitive potassium (KATP)...
Yi Fan et al.
SLAS discovery : advancing life sciences R & D, 23(7), 742-750 (2018-06-07)
Enhancing antitumor activities of the human immune system is a clinically proven approach with the advent of monoclonal antibodies recognizing programmed cell death protein-1 (PD1) receptors on immune cell surfaces. Historically, using flow cytometry as a means to assess next-generation...
Down-regulation of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene by insulin: the role of the forkhead transcription factor FKHRL1
Nadal A, et al.
The Biochemical Journal, 366(1), 289-297 (2002)
Glucagon receptor antagonism induces increased cholesterol absorption
Guan HP, et al.
Journal of Lipid Research, 56(11), 2183-2195 (2015)

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