A9210

Sigma-Aldrich

Annexin V FITC Conjugate from human placenta

Synonym(s):
Calphosbindin I, Lipocortin V, Annexin V, PAP-1
NACRES:
NA.32
Pricing and availability is not currently available.

form

buffered aqueous solution

Quality Level

extent of labeling

1-2 mol FITC per mol annexin V

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... ANXA5(308)

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Application

For detection of apoptotic cells by flow cytometry or fluorescence microscopy.

Biochem/physiol Actions

Annexins are ubiquitous homologous proteins that bind phospholipids in the presence of calcium. The cellular changes involved in the apoptotic process include loss of phospholipid asymmetry during the early stages. This phenomenon is universal and is not limited to stimulus or to mammalian cells, but also occurs in insect and plant cells. In living cells, phosphatidylserine is transported to the inside of the lipid bilayer by the Mg2+ATP dependent enzyme, aminophospholipid translocase. At the onset of apoptosis, phosphatidylserine becomes translocated to the external surface of the cell membrane. Since the movement of phosphatidylserine from the internal membrane surface to the external surface is an early indicator of apoptosis, annexin V and its conjugates that interact strongly and specifically with phosphatidylserine may be used to detect apoptosis. Annexin V conjugates can be used to detect apoptotic cells significantly earlier than DNA-based assays. Fluorescent dye labeled annexin V have applications in flow cytometry, fluorescence microscopy, and laser scanning cytometry.

Packaging

Package size based on protein content

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, containing 100 mM NaCl.

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Curcumin (diferuloylmethane) is a natural bioactive compound with many health-promoting benefits. However, its poor water solubility and bioavailability has limited curcumin’s biomedical application. In the present study, we encapsulated curcumin into liposomes, formed from natural sources (salmon lecithin), and characterized...
Chengbin Xue et al.
Bioscience, biotechnology, and biochemistry, 79(8), 1384-1390 (2015-03-31)
Nano-TiO2 has been reported to be an efficient photocatalyst, which is able to produce reactive oxygen species (ROS) under UVA irradiation. In this study, we investigated the effects of nano-TiO2 on the cytotoxicity, induction of apoptosis, and the putative pathways...

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