MilliporeSigma
All Photos(1)

Documents

A9231

Sigma-Aldrich

Acridine Orange solution

2% in H2O

Sign Into View Organizational & Contract Pricing

Select a Size

Empirical Formula (Hill Notation):
C17H19N3 · HCl
CAS Number:
Molecular Weight:
301.81
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

liquid

Quality Level

concentration

2% in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

InChI

1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3

InChI key

DPKHZNPWBDQZCN-UHFFFAOYSA-N

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A8097235474A6014
Acridine Orange base Dye content 75 %

Sigma-Aldrich

235474

Acridine Orange base

concentration

2% in H2O

concentration

10 mg/mL in H2O

concentration

-

concentration

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

General description

Acridine Orange (C.l. 46005) is an acridine dye with a ring structure and overall small planar conjugated system.

Application

Acridine orange is a DNA intercalating dye that is a recommended DNA stain for flow cytometry. It differentially stains double-stranded and single-stranded nucleic acids.

It also has the following applications-
  • staining of RNA and acid mucins
  • detecting apoptosis
  • staining blood smears to diagnose malaria
  • identifying and enumerating granulocytes in fish
  • staining eukaryotic cells to demonstrate low pH structures

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 2

1 of 2

Acriflavine suitable for fluorescence, BioReagent, ≥90% (AT)

Sigma-Aldrich

01673

Acriflavine

Matthew P Harris et al.
PLoS genetics, 4(10), e1000206-e1000206 (2008-10-04)
The genetic basis of the development and variation of adult form of vertebrates is not well understood. To address this problem, we performed a mutant screen to identify genes essential for the formation of adult skeletal structures of the zebrafish.
Dolar Pak et al.
Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes, 48(6), 512-515 (2013-03-05)
In this study we assessed the use of acridine orange as an alternative to optical density to quantify the growth of Lactobacillus bulgaricus ATCC 7517. The growth of bacteria in Lactobacillus de Man Rogosa Sharpe (MRS) medium was measured by
T V Kuznetzova et al.
Genetika, 48(4), 451-456 (2012-06-27)
A novel phenomenon of unusual selective acridine orange (AO) staining ofpericentromeric heterochromatin regions (HRs) in chromosomal preparations from tissue with known spontaneous mitotic activity (chorionic villi, placenta, embryonic tissues, bone marrow, and testes), as well as embryonic stem cells, is
Donald P Evenson
Methods in molecular biology (Clifton, N.J.), 927, 147-164 (2012-09-21)
The SCSA(®) is the pioneering assay for the detection of damaged sperm DNA and altered proteins in sperm nuclei via flow cytometry of acridine orange (AO) stained sperm. The SCSA(®) is considered to be the most precise and repeatable test
Q L Chen et al.
Talanta, 97, 48-54 (2012-07-31)
We have utilized various valving scheme to leverage purely rotation-regulated flow control to enable comprehensive cell-based bioassays (CBBs) on centrifuge-based lab-on-a-disc (LOAD). A LOAD has been developed to examine allergic degranulation from live basophils for allergens screening for the first

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service