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A9275

Sigma-Aldrich

Avidin from egg white

BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

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CAS Number:
EC Number:
MDL number:
NACRES:
NA.46

biological source

egg white

Quality Level

product line

BioUltra

assay

≥98% (SDS-PAGE)

form

lyophilized powder

specific activity

≥10 units/mg protein (E1%/280)

mol wt

glycoprotein 66 kDa
subunit 16 kDa

storage temp.

2-8°C

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This Item
A8706A9207189725
Sigma-Aldrich

A9275

Avidin from egg white

Sigma-Aldrich

A8706

Avidin from egg white

Avidin−Agarose from egg white aqueous glycerol suspension

A9207

Avidin−Agarose from egg white

Sigma-Aldrich

189725

Avidin, Egg White

assay

≥98% (SDS-PAGE)

assay

≥80% protein basis (biuret)

assay

-

assay

-

mol wt

glycoprotein 66 kDa, subunit 16 kDa

mol wt

glycoprotein 66 kDa, subunit 16 kDa

mol wt

-

mol wt

-

form

lyophilized powder

form

powder

form

aqueous glycerol suspension

form

lyophilized solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

specific activity

≥10 units/mg protein (E1%/280)

specific activity

≥12 units/mg protein (E1%/280)

specific activity

-

specific activity

-

General description

Avidin protein is a homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Egg white avidin has been used as a cross-linker for biotin-conjugated antibodies. The product has also been used as a blocking agent in ELISA applications.

Unit Definition

One unit will bind 1.0 μg of d-biotin.

Preparation Note

Affinity purified

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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