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A9275

Sigma-Aldrich

Avidin from egg white

BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

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1 MG
$39.50
2 MG
$57.25
5 MG
$109.00
10 MG
$183.20
25 MG
$366.00
100 MG
$896.76

$39.50

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All Photos(1)

Select a Size

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1 MG
$39.50
2 MG
$57.25
5 MG
$109.00
10 MG
$183.20
25 MG
$366.00
100 MG
$896.76

About This Item

CAS Number:
1405-69-2
EC Number:
215-783-6
MDL number:
MFCD00130572
UNSPSC Code:
12352203
NACRES:
NA.46

$39.50

Available to ship onMay 06, 2025Details

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Notify Me

Get notified when this item is ready to ship via email.

biological source

egg white

product line

BioUltra

assay

≥98% (SDS-PAGE)

form

lyophilized powder

specific activity

≥10 units/mg protein (E1%/280)

mol wt

glycoprotein 66 kDa
subunit 16 kDa

storage temp.

2-8°C

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General description

Avidin protein is a homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Egg white avidin has been used as a cross-linker for biotin-conjugated antibodies[2]. The product has also been used as a blocking agent in ELISA applications[3].

Unit Definition

One unit will bind 1.0 μg of d-biotin.

Preparation Note

Affinity purified

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000440771
0000440746
0000440770
0000440771
0000440770

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Lee B Sims et al.
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Q Wang et al.
The American journal of physiology, 275(5 Pt 2), R1584-R1592 (1998-10-29)
We examined the effect of endotoxemia in mice on protein and mRNA levels for the acute phase proteins complement C3 and serum amyloid A (SAA) in jejunal mucosa. Endotoxemia was induced in mice by the subcutaneous injection of 250 microg
B Steiniger et al.
The American journal of pathology, 159(2), 501-512 (2001-08-04)
We investigate the white pulp compartments of 73 human spleens and demonstrate that there are several microanatomical peculiarities in man that do not occur in rats or mice. Humans lack a marginal sinus separating the marginal zone (MZ) from the
Roland Hager et al.
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Lee B Sims et al.
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