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A9309

Sigma-Aldrich

Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
HRP Goat Anti-Mouse IgG (Fc specific)
MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot: 1:40,000-1:80,000 using total cell extract of HeLa cells

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
A0168A2554A3682
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

goat

biological source

goat

biological source

goat

biological source

goat

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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General description

Goat polyclonal Mouse IgG (Fc specific)–Peroxidase antibody is determined against normal mouse serum, mouse IgG (whole molecule), the Fc fragment of mouse IgG and the Fab fragment of mouse IgG, the conjugate is specific for mouse IgG and shows no reaction with the Fab fragment of mouse IgG. The conjugate only shows reactivity with mouse IgG (whole molecule) and the Fc fragment of mouse IgG, when tested in ELISA. The conjugate shows no reaction with the Fab fragment of mouse IgG, human IgG, IgA, IgM, or rat IgG in ELISA.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum.

Application

Anti-Mouse IgG (Fc specific)-Peroxidase antibody has been used:
  • in immunoblotting
  • in immunohistochemistry
  • in enzyme linked immunosorbent assay (ELISA)
  • in agar block precipitin titration
  • in western blotting
  • in assessment of in vitro poly(ADP-ribose)polymerase (PARP) activity

Indirect ELISA was performed on sera from mice immunized to SV40-Tag using HRP-conjugated goat anti-mouse Fc specific IgG. Antibody was used at a 1:1000 dilution for 30 minutes at 37 degrees.
Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.

Biochem/physiol Actions

IgG antibodies help in neutralizing the effects caused by viruses and toxins.
IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu Red into detectable chromophores, light-emitters or fluorescers, respectively.

Other Notes

Antibody adsorbed with human IgG and rat serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Preparation Note

Adsorbed to reduce background staining with human or rat samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Legal Information

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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