A9309

Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Synonym(s): HRP Goat Anti-Mouse IgG (Fc specific)
• MDL number: MFCD00162629
• NACRES: NA.46

Properties

• biological source: goat
• Quality Level: 200
• conjugate: peroxidase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• technique(s): direct ELISA: 1:40,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200; western blot: 1:40,000-1:80,000 using total cell extract of HeLa cells
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Goat polyclonal Mouse IgG (Fc specific)–Peroxidase antibody is determined against normal mouse serum, mouse IgG (whole molecule), the Fc fragment of mouse IgG and the Fab fragment of mouse IgG, the conjugate is specific for mouse IgG and shows no reaction with the Fab fragment of mouse IgG. The conjugate only shows reactivity with mouse IgG (whole molecule) and the Fc fragment of mouse IgG, when tested in ELISA. The conjugate shows no reaction with the Fab fragment of mouse IgG, human IgG, IgA, IgM, or rat IgG in ELISA.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum.

Application

Anti-Mouse IgG (Fc specific)-Peroxidase antibody has been used:

• in immunoblotting
• in immunohistochemistry
• in enzyme linked immunosorbent assay (ELISA)
• in agar block precipitin titration
• in western blotting
• in assessment of in vitro poly(ADP-ribose)polymerase (PARP) activity

Indirect ELISA was performed on sera from mice immunized to SV40-Tag using HRP-conjugated goat anti-mouse Fc specific IgG. Antibody was used at a 1:1000 dilution for 30 minutes at 37 degrees.

Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.

Packaging

1 mL

Biochem/physiol Actions

IgG antibodies help in neutralizing the effects caused by viruses and toxins.

IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu^™ Red into detectable chromophores, light-emitters or fluorescers, respectively.

Other Notes

Antibody adsorbed with human IgG and rat serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Preparation Note

Adsorbed to reduce background staining with human or rat samples.

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Legal Information

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable