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A9316

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

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Synonym(s):
Goat Anti-Mouse IgG (whole molecule)−AP
MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

technique(s)

direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
A3562A3688A5153
technique(s)

direct ELISA: 1:30,000, western blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:7,000-1:21,000

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

biological source

goat

biological source

goat

biological source

goat

biological source

goat

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

Goat polyclonal anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody is reactive with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM. Cross reactivity of the antibody conjugate is determined by ELISA. The conjugate shows no reactivity with rat IgG or rat serum proteins.
Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant immunoglobulin present in human serum. The four classes of IgG are IgG1, IgG2, IgG3, and IgG4.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

Application

Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in goat has been used as a secondary antibody in:
  • histological studies in heart and liver grafts
  • immunohistochemical studies of gangliosides and immunostaining of lymphoid organs
  • western blot detection in blood samples
Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at μg/ml in TBS/Tween containing final concentration of 0.5M NaCl.
The level of antibodies specific to P. aeuginosa present in immunized mouse sera was determined by ELISA using alkaline phosphatase-conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:1000.

Biochem/physiol Actions

IgG1 deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection.

Other Notes

Antibody adsorbed with rat serum proteins.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with rat samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Alcohol administration blocks stress-induced impairments in memory and anxiety, and alters hippocampal neurotransmitter receptor expression in male rats
Gomez JL, et al.
Hormones and Behavior, 63(4), 659-666 (2013)
Lei Guo et al.
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, 10(6), 743-747 (2004-05-27)
A member of the costimulatory molecule family, inducible costimulator (ICOS), is expressed on activated T cells and plays a critical role in their primary activation and cytokine production. ICOS is involved in different immune phenomena, such as Th1-mediated autoimmune disease
Significant enhancement by anti-ICOS antibody of suboptimal tacrolimus immunosuppression in rat liver transplantation
Guo L, et al.
Liver Transplantation, 10(6), 743-747 (2004)
Simultaneous immunohistochemical detection of gangliosides and neuronal markers in paraformaldehyde-fixed nervous tissues by acetone etching
Sakumoto Y, et al.
Archives of Histology and Cytology, 72(2), 77-90 (2009)
The microstructure of secondary lymphoid organs that support immune cell trafficking
Matsuno K, et al.
Archives of Histology and Cytology, 73(1), 1-21 (2010)

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