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A9325

Sigma-Aldrich

L-Alanine 4-nitroanilide hydrochloride

protease substrate, ≥99% (TLC), powder

Synonym(s):

Ala-4-nitroanilide, H-Ala-pNA

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100 MG
$64.20
500 MG
$168.00
1 G
$248.00
5 G
$935.00

About This Item

Empirical Formula (Hill Notation):
C9H11N3O3 · HCl
CAS Number:
31796-55-1
Molecular Weight:
245.66
Beilstein/REAXYS Number:
6303731
EC Number:
250-811-0
MDL number:
MFCD00039088
UNSPSC Code:
12352204
eCl@ss:
32160406
PubChem Substance ID:
24891436
NACRES:
NA.83

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$64.20

In StockDetails

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Product Name

L-Alanine 4-nitroanilide hydrochloride, protease substrate

Quality Level

200

assay

≥99% (TLC)

form

powder

solubility

water: 50 mg/mL, clear to very slightly hazy

storage temp.

−20°C

SMILES string

Cl[H].C[C@H](N)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C9H11N3O3.ClH/c1-6(10)9(13)11-7-2-4-8(5-3-7)12(14)15;/h2-6H,10H2,1H3,(H,11,13);1H/t6-;/m0./s1

InChI key

YEXRLSXNWLNHQR-RGMNGODLSA-N

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This Item
M4765A3401454454
L-Alanine 4-nitroanilide hydrochloride protease substrate

A9325

L-Alanine 4-nitroanilide hydrochloride

N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide elastase substrate

M4765

N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide

Ala-Ala-Phe-7-amido-4-methylcoumarin protease substrate

A3401

Ala-Ala-Phe-7-amido-4-methylcoumarin

Methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide Soluble, specific substrate for human leukocyte elastase.

454454

Methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide

assay

≥99% (TLC)

assay

≥98% (HPLC)

assay

≥98% (TLC)

assay

≥98% (HPLC)

solubility

water: 50 mg/mL, clear to very slightly hazy

solubility

DMF: soluble 25 mg/mL, clear, colorless to light yellow

solubility

ethanol: 20 mg/mL, clear, colorless to light yellow

solubility

ethanol: 1 mg/mL, DMF: 5 mg/mL, DMSO: 5 mg/mL

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

100

form

powder

form

powder

form

powder

form

solid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

L-Alanine 4-nitroanilide hydrochloride has been used to determine the activity of aminopeptidase in the intestinal segments and small intestine (jejunum and ileum).[1]

Biochem/physiol Actions

L-Alanine-4-nitroanilide is used as a substrate to determine alanine aminopeptidase (AAP) activity. It is mainly chosen as a substrate, due to its relative specificity, higher hydrolysis rate and better solubility.[2]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK5641
BCCD6991
BCBG9297V
BCBV4313
BCBQ8433V

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