A9434

Sigma-Aldrich

Ammonium chloride

for molecular biology, suitable for cell culture, ≥99.5%

Synonym(s):
Salmiac
Linear Formula:
NH4Cl
CAS Number:
Molecular Weight:
53.49
Beilstein/REAXYS Number:
4371014
EC Number:
MDL number:
eCl@ss:
38050207
PubChem Substance ID:
NACRES:
NA.31

Quality Level

grade

for molecular biology

vapor density

1.9 (vs air)

vapor pressure

1 mmHg ( 160.4 °C)

assay

≥99.5%

form

powder or crystals

application(s)

cell culture | mammalian: suitable

mp

340 °C (subl.) (lit.)

anion traces

phosphate (PO43-): ≤2 ppm
sulfate (SO42-): ≤20 ppm

cation traces

Fe: ≤2 ppm
heavy metals (as Pb): ≤5 ppm

foreign activity

DNase, RNase, protease, none detected

SMILES string

N.Cl

InChI

1S/ClH.H3N/h1H;1H3

InChI key

NLXLAEXVIDQMFP-UHFFFAOYSA-N

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Related Categories

Application

Ammonium chloride has been used:
  • as an inhibitor of endosome acidification in vero cells
  • as an autophagy inhibitor in vascular smooth muscle cell (VSMC)
  • in the depletion and lysis of erythrocytes in testis parenchyma homogenate

Packaging

500 g in poly bottle
1 kg in poly bottle

Biochem/physiol Actions

Ammonium chloride is one of the three principal components of the nitrogen cycle. Ammonium chloride administration promotes creatinine and urea clearance andinduces metabolic acidosis in mice. Excess of ammonium chloride inhibits Kreb′s pathway in brain and depletes ATP in astrocytes. Ammonium chloride inhibits acidification in the endosome-lysosome system. Ammonium chloride is a hemolysing agent used for the lysis of erythrocytes.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

hazcat

Acute Tox. 4 Oral - Eye Irrit. 2

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Nrf2 Activation Provides Atheroprotection in Diabetic Mice Through Concerted Upregulation of Antioxidant, Anti-inflammatory, and Autophagy Mechanisms
Lazaro I, et al.
Frontiers in Pharmacology, 9, 118-136 (2018)
Effect of ammonium chloride and dietary phosphorus in the azotaemic rat. I. Renal function and biochemical changes
Jara A, et al.
Nephrology, Dialysis, and Transplantation, 19(8), 1986-1992 (2004)
Aqueous ammonia and ammonium chloride hydrates: Principal infrared spectra
Max JJ and Chapados C
Journal of Molecular Structure, 1046, 124-135 (2013)
Inhibition of endosome-lysosome system acidification enhances porcine circovirus 2 infection of porcine epithelial cells
Misinzo G, et al.
Journal of Virology, 82(3), 1128-1135 (2008)
Infectious bronchitis virus entry mainly depends on clathrin mediated endocytosis and requires classical endosomal/lysosomal system
Wang H, et al.
Virology, 528, 118-136 (2019)
Protocols
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
Read More

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