A9543

Bovine Serum Albumin

heat shock fraction, low endotoxin, pH 7, ≥98%

• Synonym(s): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• NACRES: NA.27

Properties

• biological source: bovine
• Quality Level: 200
• Assay: ≥98%
• form: lyophilized powder
• mol wt: ~66 kDa
• purified by: heat shock fractionation
• packaging: poly bottle of
• origin: USA origin
• technique(s): ELISA: suitable; electrophoresis: suitable; western blot: suitable
• impurities: ≤1.0 EU/mg endotoxin
• loss: ≤5%
• pH: 7
• solubility: water: soluble (40 mg/ml)
• UniProt accession no.: P02769
• foreign activity: BT virus, none detected; VSV virus, none detected
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

Application

This A9543 BSA product has been used in various systems, including:

• NRK-52E rat kidney epithelioid cells: Ochiai, H. et al., J. Virol., 67(11), 6811-6814 (1993)
• Neonatal porcine pancreatic islets: Ellis, C.E. et al., Islets, 5(5), 216-225 (2013)
• Mouse:

Lee, D. et al., Am. J. Physiol. Renal Physiol., 304(7), F1009-F1019 (2013)
Girbl, T. et al., Immunity, 49(6), 1062-1076.e6 (2018)

• iPSC cell line: Martin, H.C. et al., Science, 362(6419), 1161-1164 (2018).

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

• Heat shock fractionated
• Low endotoxin

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves