A9543

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, low endotoxin, pH 7, ≥98%

Synonym(s):
Albumin bovine serum, Bovine albumin, BSA
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

Quality Level

biological source

bovine

assay

≥98%

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

application(s)

ELISA: suitable
electrophoresis: suitable
western blot: suitable

impurities

≤1.0 EU/mg endotoxin

loss

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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Application

This A9543 BSA product has been used in various systems, including:
  • NRK-52E rat kidney epithelioid cells: Ochiai, H. et al., J. Virol., 67(11), 6811-6814 (1993)
  • Neonatal porcine pancreatic islets: Ellis, C.E. et al., Islets, 5(5), 216-225 (2013)
  • Mouse:
Lee, D. et al., Am. J. Physiol. Renal Physiol., 304(7), F1009-F1019 (2013)
Girbl, T. et al., Immunity, 49(6), 1062-1076.e6 (2018)
  • iPSC cell line: Martin, H.C. et al., Science, 362(6419), 1161-1164 (2018).

Packaging

5, 25 g in poly bottle

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Heat shock fractionated
  • Low endotoxin

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Marcos V A S Navarro et al.
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