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A9544

Sigma-Aldrich

Anti-Human IgG (Fc specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti Human Igg, Anti-Human IgG Antibody - Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody produced in goat, Anti-Human Igg, anti human IgG antibody
MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:55,000
dot blot: 1:40,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
A3187A8542A1543
conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

direct ELISA: 1:55,000, dot blot: 1:40,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:40,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot (chemiluminescent): 1:30,000

technique(s)

direct ELISA: 1:20,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot (chemiluminescent): 1:30,000

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 percent of the total immunoglobulin consists of IgG. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody is specific for human IgG and human IgG, Fc fragment. The product does not react with human IgG, Fab fragment, light chains, IgA, and IgM. Additionally, the antibody does not bind to mouse and rat IgG. Goat anti-human IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.

Specificity

Specificity of the Anti-Human IgG is determined prior to conjugation by ELISA. The conjugate is specific for human IgG and human IgG, Fc fragment. Cross reactivity of the antibody-conjugate is also determined by ELISA. The conjugate shows no reactivity with human IgG, Fab fragment, human light chains, human IgA, human IgM or with mouse IgG and rat IgG.
Binds human IgG; does not bind other human Igs.
Provides reduced background with mouse or rat samples.

Immunogen

Human IgG, Fc fragment

Application

A modified monoclonal antibody specific immobilization of platelet antigens (MAIPA) assay was performed on human platelet eluates using alkaline phosphatase-conjugated goat anti-human, Fc specific, antibody as the secondary with a 30 minute incubation.
Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody is suitable for use in ELISA and western blot at 1:5000 dilutions. The product can also be used for dot blot (1:40,000) and immunohistochemistry (1:150 using formalin-fixed, paraffin-embedded sections).

Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody has been used in
  • quantitative sandwich ELISA
  • α-GalC ELISA
  • ELISA assays
  • ELISAs for IgG subclasses

Biochem/physiol Actions

Immunoglobulin G (IgG) regulates immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Immunoglobulin G (IgG) mainly participates in hypersensitivity type II and type III.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% BSA, 1 mM MgCl2 and 15 mM sodium azide

Storage and Stability

Store at 2-8 °C. Do Not Freeze.

Other Notes

No cross-reaction with mouse and rat IgG.
Working dilutions should be determined by titration assay. Due to differences in assay systems, these titers may not reflect the user′s actual working dilution.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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A Juhász et al.
Journal of medical virology, 64(4), 526-530 (2001-07-27)
Sera of blood donors were investigated by a peptide ELISA and indirect immunofluorescence assay to assess the prevalence of HHV-8 infection in the Hungarian population. A 14 amino acid long synthetic oligopeptide from the carboxyterminus of orf65/small virus capsid antigen
Cross-reactive phage-displayed mimotopes lead to the discovery of mimicry between HSV-1 and a brain-specific protein.
Cortese I, et al.
Journal of Neuroimmunology, 113(1), 119-128 (2001)
Human interleukin 10 induces naive surface immunoglobulin D+ (sIgD+) B cells to secrete IgG1 and IgG3.
Briere F, et al.
The Journal of Experimental Medicine, 179(2), 757-762 (1994)
Long-lived plasma cells from human small intestine biopsies secrete immunoglobulins for many weeks in vitro.
Mesin L, et al.
Journal of Immunology, 1003181-1003181 (2011)
Ming Hou et al.
European journal of haematology, 70(6), 353-357 (2003-05-21)
The treatment of chronic idiopathic thrombocytopenic purpura (ITP) is difficult in those unresponsive to corticosteroids and/or splenectomy. We attempted to induce durable response in 21 patients with refractory ITP by applying mycophenolate mofetil (MMF) (1.5-2.0 g/d), a novel immunosuppressive agent.

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